Glutamate-Ammonia Ligase (GLUL) (N-Term) antibody

Antigen: Glutamate-Ammonia Ligase (GLUL)

Synonyms: glul, MGC64262, GS, GLNS, PIG43, PIG59, Glns, GLUL, MGC128403, xgs, F15E12.14, F15E12_14, GLUTAMINE SYNTHETASE

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN390879

Additional Information

Alternative name: GLUL

Gene ID: 2752

UniProt: P15104

Immunogen: This GLUL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 76~105 amino acids from the N-terminal region of human GLUL.

Isotype: Ig

Description: Other names: Glutamine synthetase, GS, Glutamate--ammonia ligase, GLNS, GLUL

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This GLUL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 76~105 amino acids from the N-terminal region of human GLUL.

Molecular Weight: 42064 DA

Comments:

Background: GLUL belongs to the glutamine synthetase family. It catalyzes the synthesis of glutamine from glutamate and ammonia. Glutamine is a main source of energy and is involved in cell proliferation, inhibition of apoptosis, and cell signaling.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Protein Modifications, Neurology

Restrictions: For Research Use only

Images

Western blot analysis of GLUL Antibody (N-term) (ABIN390879) in mouse cerebellum tissue lysates (35ug/lane). GLUL (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human testis tissue reacted with GLUL Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Publications

Product: Di Tommaso, Destro, Seok et al.: "The application of markers (HSP70 GPC3 and GS) in liver biopsies is useful for detection of hepatocellular carcinoma." in: Journal of hepatology, Vol. 50, Issue 4, pp. 746-54, 2009 (PubMed).