Fat Mass and Obesity-Associated (FTO) (N-Term) antibody

Antigen: Fat Mass and Obesity-Associated (FTO)

Synonyms: AW743446, mKIAA1752, MGC5149, KIAA1752, RGD1305121, DKFZp459H168

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN390964

Additional Information

Alternative name: FTO

Gene ID: 79068

UniProt: Q9C0B1

Immunogen: This FTO antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of human FTO.

Isotype: Ig

Description: Other names: Protein fto, Fat mass and obesity-associated protein, KIAA1752, FTO

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This FTO antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of human FTO.

Molecular Weight: 58282 DA

Comments:

Background: The precise function of FTO remains to be determined.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Restrictions: For Research Use only

Images

Western blot analysis of FTO Antibody (N-term) (ABIN390964) in NCI-H460 cell line lysates (35ug/lane). FTO (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human brain tissue reacted with FTO Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Flow cytometric analysis of NCI-H292 cells using FTO Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Scott, Mohlke, Bonnycastle et al.: "A genome-wide association study of type 2 diabetes in Finns detects multiple susceptibility variants." in: Science (New York, N.Y.), Vol. 316, Issue 5829, pp. 1341-5, 2007 (PubMed).