FTO antibody (N-Term)

Catalog No. ABIN6254099

Product Details anti-FTO Antibody

Target: FTO (Fat Mass and Obesity-Associated (FTO))

Binding Specificity: N-Term

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FTO antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Predicted Reactivity: bovine, canine, horse, non-human primate, sheep

Purification: Protein G purified culture supernatant

Immunogen: Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH

Clone: 5-2H10

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Storage: -20 °C

References for anti-FTO antibody (ABIN6254099)

Mao, Dong, Liu, Guo, Ma, Shen, Qian: "m6A in mRNA coding regions promotes translation via the RNA helicase-containing YTHDC2." in: Nature communications, Vol. 10, Issue 1, pp. 5332, (2020) (PubMed).

Liu, Zhou, Mao, Ji, Qian: "Programmable RNA N6-methyladenosine editing by CRISPR-Cas9 conjugates." in: Nature chemical biology, Vol. 15, Issue 9, pp. 865-871, (2019) (PubMed).

Zhou, Wan, Shu, Mao, Liu, Yuan, Zhang, Hess, Brüning, Qian: "N6-Methyladenosine Guides mRNA Alternative Translation during Integrated Stress Response." in: Molecular cell, Vol. 69, Issue 4, pp. 636-647.e7, (2019) (PubMed).

Wang, Li, Yue, Wang, Kumar, Wechsler-Reya, Zhang, Ogawa, Kellis, Duester, Zhao: "N6-methyladenosine RNA modification regulates embryonic neural stem cell self-renewal through histone modifications." in: Nature neuroscience, Vol. 21, Issue 2, pp. 195-206, (2019) (PubMed).

Yu, Chen, Huang, Zhu, Song, Zhu, Park, Ji: "Dynamic m6A modification regulates local translation of mRNA in axons." in: Nucleic acids research, Vol. 46, Issue 3, pp. 1412-1423, (2019) (PubMed).

Zhou, Wan, Gao, Zhang, Jaffrey, Qian: "Dynamic m(6)A mRNA methylation directs translational control of heat shock response." in: Nature, Vol. 526, Issue 7574, pp. 591-4, (2015) (PubMed).

Target Details for FTO

Target: FTO (Fat Mass and Obesity-Associated (FTO))

Alternative Name: FTO (FTO Products)

Synonyms: AW743446 antibody, mKIAA1752 antibody, RGD1305121 antibody, FTO, alpha-ketoglutarate dependent dioxygenase antibody, fat mass and obesity associated antibody, fat mass and obesity associated L homeolog antibody, FTO antibody, Fto antibody, fto.L antibody

Molecular Weight: '58 kDa

Gene ID: 79068

UniProt: Q9C0B1