Adrenergic, Beta, Receptor Kinase 2 (ADRBK2) (C-Term) antibody

Antigen: Adrenergic, Beta, Receptor Kinase 2 (ADRBK2)

Synonyms: GRK3, BARK2, Grk3, si:dz221d18.1

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Primate

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN390986

Additional Information

Alternative name: GRK3

Gene ID: 157

UniProt: P35626

Immunogen: This GRK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 639~669 amino acids from the C-term region of human GRK3.

Isotype: Ig

Description: Other names: G-protein coupled receptor kinase 3, Beta-adrenergic receptor kinase 2, Beta-ARK-2

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This GRK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 639~669 amino acids from the C-term region of human GRK3.

Molecular Weight: 79677 DA

Comments:

Background: The beta-adrenergic receptor kinase specifically phosphorylates the agonist-occupied form of the beta-adrenergic and related G protein-coupled receptors. Overall, the ADRBK2 enzyme, also known as GRK3, has 85% amino acid similarity with ADRBK1, with the protein kinase catalytic domain having 95% similarity. The ADRBK2 mRNA is approximately 8 kilobases with a distribution similar to that of ADRBK1. These data suggest the existence of a family of receptor kinases which may serve broadly to regulate receptor function.

Application Details

Application Notes: The suggested dilution will be: ELISA~~1:1,000 Western blotting~~1:100~500 Flow cytometry

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

The anti-GRK3 Pab (ABIN390986) is used in Western blot to detect GRK3 in SK-OV-3 cell lysate (Lane 1) and mouse heart tissue lysate (Lane 2).

Publications

Rao, Rapoport, Kim: "Decreased GRK3 but not GRK2 expression in frontal cortex from bipolar disorder patients." in: The international journal of neuropsychopharmacology / official scientific journal of the Collegium Internationale Neuropsychopharmacologicum (CINP), Vol. 12, Issue 6, pp. 851-60, 2009 (PubMed).

Product: Benovic, Onorato, Arriza et al.: "Cloning, expression, and chromosomal localization of beta-adrenergic receptor kinase 2. A new member of the receptor kinase family." in: The Journal of biological chemistry, Vol. 266, Issue 23, pp. 14939-46, 1991 (PubMed).

Calabrese, Sallese, Stornaiuolo et al.: "Chromosome mapping of the human arrestin (SAG), beta-arrestin 2 (ARRB2), and beta-adrenergic receptor kinase 2 (ADRBK2) genes." in: Genomics, Vol. 23, Issue 1, pp. 286-8, 1995 (PubMed).

Parruti, Ambrosini, Sallese et al.: "Molecular cloning, functional expression and mRNA analysis of human beta-adrenergic receptor kinase 2." in: Biochemical and biophysical research communications, Vol. 190, Issue 2, pp. 475-81, 1993 (PubMed).