|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Adrenergic, Beta, Receptor Kinase 2 (ADRBK2) (C-Term) antibody
|Synonyms||GRK3, BARK2, Grk3, si:dz221d18.1|
Alternatives Western Blotting (WB), ELISA
|4 references available|
|Quantity||0.1 mg (0.25 mg/ml) (Variants)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||This GRK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 639~669 amino acids from the C-term region of human GRK3.|
|Description||Other names: G-protein coupled receptor kinase 3, Beta-adrenergic receptor kinase 2, Beta-ARK-2|
|Characteristics||Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This GRK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 639~669 amino acids from the C-term region of human GRK3.|
|Molecular Weight||79677 DA|
Background: The beta-adrenergic receptor kinase specifically phosphorylates the agonist-occupied form of the beta-adrenergic and related G protein-coupled receptors. Overall, the ADRBK2 enzyme, also known as GRK3, has 85% amino acid similarity with ADRBK1, with the protein kinase catalytic domain having 95% similarity. The ADRBK2 mRNA is approximately 8 kilobases with a distribution similar to that of ADRBK1. These data suggest the existence of a family of receptor kinases which may serve broadly to regulate receptor function.
|Application Notes||The suggested dilution will be: ELISA~~1:1,000 Western blotting~~1:100~500 Flow cytometry|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure|
|Restrictions||For Research Use only|
|The anti-GRK3 Pab (ABIN390986) is used in Western blot to detect GRK3 in SK-OV-3 cell lysate (Lane 1) and mouse heart tissue lysate (Lane 2).|
Rao, Rapoport, Kim: "Decreased GRK3 but not GRK2 expression in frontal cortex from bipolar disorder patients." in: The international journal of neuropsychopharmacology / official scientific journal of the Collegium Internationale Neuropsychopharmacologicum (CINP), Vol. 12, Issue 6, pp. 851-60, 2009 (PubMed).
Benovic, Onorato, Arriza et al.: "Cloning, expression, and chromosomal localization of beta-adrenergic receptor kinase 2. A new member of the receptor kinase family." in: The Journal of biological chemistry, Vol. 266, Issue 23, pp. 14939-46, 1991 (PubMed).
Calabrese, Sallese, Stornaiuolo et al.: "Chromosome mapping of the human arrestin (SAG), beta-arrestin 2 (ARRB2), and beta-adrenergic receptor kinase 2 (ADRBK2) genes." in: Genomics, Vol. 23, Issue 1, pp. 286-8, 1995 (PubMed).
Parruti, Ambrosini, Sallese et al.: "Molecular cloning, functional expression and mRNA analysis of human beta-adrenergic receptor kinase 2." in: Biochemical and biophysical research communications, Vol. 190, Issue 2, pp. 475-81, 1993 (PubMed).
|Reactivities||Human (34), Rat (Rattus) (26), Mouse (Murine) (25), Chicken (20), Cow (Bovine) (20), Dog (Canine) (19), Horse (Equine) (18), Pig (Porcine) (18), Sheep (Ovine) (18), Cat (Feline) (1), Zebrafish (1)|
|Applications||Western Blotting (WB) (20), Immunofluorescence (IF) (16), ELISA (15), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunohistochemistry (IHC) (3), Immunoprecipitation (IP) (2), Immunoelectron Microscopy (IEM) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE-Cy3 (1), PE-Cy5 (1), PE-Cy5.5 (1), PE-Cy7 (1), Un-conjugated (1)|
|Epitopes||C-Term (3), N-Term (3), Internal Region (1), pThr284 (1)|