PRKD3 antibody (AA 352-384)

Catalog No. ABIN391014

Cited in 1 publication. The Rabbit Polyclonal anti-PRKD3 antibody (Clone RB1047) (ABIN391014) specifically detects PRKD3 in WB and IHC (p). The antibody is reactive with Human samples.

Quick Overview for PRKD3 antibody (AA 352-384) (ABIN391014)

Target: PRKD3 (Protein Kinase D3 (PRKD3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRKD3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB1047

Product Details anti-PRKD3 Antibody

Binding Specificity: AA 352-384

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This PKC-nu antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 352-384 amino acids from human PKC-nu.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-PRKD3 antibody (ABIN391014)

Chen, Deng, Singh, Wang: "Protein kinase D3 (PKD3) contributes to prostate cancer cell growth and survival through a PKCepsilon/PKD3 pathway downstream of Akt and ERK 1/2." in: Cancer research, Vol. 68, Issue 10, pp. 3844-53, (2008) (PubMed).

Target Details for PRKD3

Target: PRKD3 (Protein Kinase D3 (PRKD3))

Alternative Name: PKC nu

Background: Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. PKC nu is one of the PKC family members. This kinase can be activated rapidly by the agonists of G protein-coupled receptors. It resides in both cytoplasm and nucleus, and its nuclear accumulation is found to be dramatically enhanced in response to its activation. This kinase can also be activated after B-cell antigen receptor (BCR) engagement, which requires intact phopholipase C gamma and the involvement of other PKC family members.

Molecular Weight: 100471

Gene ID: 23683

NCBI Accession: NP_005804

UniProt: O94806