Pyruvate Kinase, Muscle (PKM2) (C-Term) antibody

Antigen: Pyruvate Kinase, Muscle (PKM2)

Synonyms: PK3, PKM, TCB, OIP3, CTHBP, THBP1, MGC3932, Pk3, Pk-2, Pk-3, AA414905, AL024370, AL024424, PKM12, Pyk, DmelCG7070, CG7070, pkm2, cb1029, fj33f03, wu:fj33f03, MGC53388, PKM2, PYK3b, PYK3c, PK-S

Epitope: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus), Primate

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391053

Additional Information

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Alternative name: Pyruvate Kinase (PKM2) (C-term)

Gene ID: 5315

Swiss-Prot: P14786

Immunogen: This PKM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 483~513 amino acids from the C-term region of human PKM2.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: Pyruvate kinase, isozymes M1/M2, Pyruvate kinase muscle isozyme, Cytosolic thyroid hormone-binding protein, CTHBP, THBP1, PKM

Specificity: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-term region of human PKM2.

Molecular Weight: 57805 DA

Application Details

Application Notes: The suggested dilution will be: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~1:100 Immunofluorescence~~1:10~50 Flow cytometry

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Stem Cells, Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-h PKM2-N491 Pab (ABIN391053) in Ramos cell line lysates (35ug/lane). hPKM2-N491(arrow) was detected using the purified Pab (1:60dilution).

Pyruvate Kinase (PKM2) Antibody (C-term) (ABIN391053) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Pyruvate Kinase (PKM2) Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

(Top) Negative control of hela cells without Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L).Alexa-Fluor-546 emits orange fluorescence. (Bottom) Immunofluorescence analysis of Pyruvate Kinase (PKM2) Antibody (C-term) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L).