PKM2 antibody (AA 476-505)

Catalog No. ABIN3032237

Product Details anti-PKM2 Antibody

Target: PKM2 (Pyruvate Kinase M2 (PKM2))

Binding Specificity: AA 476-505

Reactivity: Human, Mouse, Rat, Primate

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKM2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Purification: Purified

Immunogen: A portion of amino acids 476-505 from the human protein was used as the immunogen for this PKM2 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the PKM2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Immunofluorescence: 1:200,Western blot: 1:1000,IHC (Paraffin): 1:50-1:1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS pH 7.4 with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the PKM2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for PKM2

Target: PKM2 (Pyruvate Kinase M2 (PKM2))

Alternative Name: PKM2 (PKM2 Products)

Synonyms: CTHBP antibody, OIP3 antibody, PK3 antibody, PKM2 antibody, TCB antibody, THBP1 antibody, AA414905 antibody, AL024370 antibody, AL024424 antibody, Pk-2 antibody, Pk-3 antibody, Pk3 antibody, Pkm2 antibody, PKM12 antibody, PKM antibody, pyruvate kinase M1/2 antibody, pyruvate kinase, muscle antibody, PKM antibody, Pkm antibody

Background: PKM2 is a glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival. [UniProt]

UniProt: P14618

Pathways: Warburg Effect