DCK antibody (C-Term)

Catalog No. ABIN391125

Cited in 2+ publications. The Rabbit Polyclonal anti-DCK antibody (Clone RB05276) (ABIN391125) specifically detects DCK in WB, IF, IP and IHC (p). The antibody is reactive with Human and Mouse samples.

Quick Overview for DCK antibody (C-Term) (ABIN391125)

Target: DCK (Deoxycytidine Kinase (DCK))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DCK antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB05276

Product Details anti-DCK Antibody

Binding Specificity: AA 171-200, C-Term

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This DCK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 171-200 amino acids from the C-terminal region of human DCK.

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:10~50. IP: 1:500~1000. WB: 1:1000. IHC-P: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-DCK antibody (ABIN391125)

Hartford, Duan, Delaney, Mi, Kistner, Lamba, Huang, Dolan: "Population-specific genetic variants important in susceptibility to cytarabine arabinoside cytotoxicity." in: Blood, Vol. 113, Issue 10, pp. 2145-53, (2009) (PubMed).

Lamba, Crews, Pounds, Schuetz, Gresham, Gandhi, Plunkett, Rubnitz, Ribeiro: "Pharmacogenetics of deoxycytidine kinase: identification and characterization of novel genetic variants." in: The Journal of pharmacology and experimental therapeutics, Vol. 323, Issue 3, pp. 935-45, (2007) (PubMed).

Target Details for DCK

Target: DCK (Deoxycytidine Kinase (DCK))

Alternative Name: DCK

Background: Deoxycytidine kinase is responsible for the phosphorylation of several deoxyribonucleosides and their analogs. Deficiency of this enzyme activity is associated with resistance to antiviral and anticancer chemotherapeutic agents, whereas increased enzyme activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. It is the rate limiting enzyme in the activation of many important anticancer and retroviral drugs and its activity is often decreased in cells that are resistant to cytosine arabinoside.

Molecular Weight: 30519

Gene ID: 1633

NCBI Accession: NP_000779

UniProt: P27707