Pantothenate Kinase 3 (PANK3) (Center) antibody

Antigen: Pantothenate Kinase 3 (PANK3)

Synonyms: FLJ12899, MGC16863, MGC38148, MGC84572, MGC142994, fb79e08, zgc:63556, wu:fb79e08

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391240

Additional Information

Alternative name: PANK3

Gene ID: 79646

UniProt: Q9H999

Immunogen: This PANK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 158~188 amino acids from the Center region of human PANK3.

Isotype: Ig

Description: Other names: Pantothenate kinase 3, Pantothenic acid kinase 3, hPanK3,

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This PANK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 158~188 amino acids from the Center region of human PANK3.

Molecular Weight: 41094 DA

Comments:

Background: PANK3 belongs to the pantothenate kinase family. Pantothenate kinase is a key regulatory enzyme in the biosynthesis of coenzyme A (CoA) in bacteria and mammalian cells. It catalyzes the first committed step in the universal biosynthetic pathway leading to CoA and is itself subject to regulation through feedback inhibition by CoA. This family member is expressed most abundantly in the liver.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Metabolism, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-PANK3 (ABIN391240) in HepG2 cell line lysate (35ug/lane).PANK3(arrow) was detected using the purified Pab (1:50 dilution).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Zhou, Westaway, Levinson et al.: "A novel pantothenate kinase gene (PANK2) is defective in Hallervorden-Spatz syndrome." in: Nature genetics, Vol. 28, Issue 4, pp. 345-9, 2001 (PubMed).