Mitogen-Activated Protein Kinase 14 (MAPK14) (Thr180,Tyr182) antibody

Antigen: Mitogen-Activated Protein Kinase 14 (MAPK14)

Synonyms: RK, p38, EXIP, Mxi2, CSBP1, CSBP2, CSPB1, PRKM14, PRKM15, SAPK2A, p38ALPHA, Hog, CRK1, CSBP, Exip, Csbp1, Csbp2, Prkm14, Prkm15, Sapk2A, p38Hog, p38alpha, MGC105413, p38b, zp38b, MAPK14, MGC142910, 186F5S, BG:DS00797.3, D-p38, D-p38b, Dm p38b, Dp38, ESTS:186F5S, Mpk34C, anon-sts23, p38 MAPK, p38 beta, p38B, p38beta, DmelCG7393, CG7393

Binding Site: Thr180,Tyr182

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391334

Additional Information

Alternative name: MAPK14

Gene ID: 1432

UniProt: Q16539

Immunogen: This MAPK14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 168~197 amino acids surrounding T180 of human MAPK14.

Isotype: Ig

Description: Other names: Mitogen-activated protein kinase 14, Mitogen-activated protein kinase p38alpha, MAP kinase p38alpha, Cytokine suppressive anti-inflammatory drug binding protein, CSAID binding protein, CSBP, MAX-interacting protein 2, MAP kinase MXI2, SAPK2A

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This MAPK14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 168~197 amino acids surrounding T180 of human MAPK14.

Molecular Weight: 41293 DA

Comments:

Background: MAPK14 is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response.

Synonyms: RK, p38, EXIP, Mxi2, CSBP1, CSBP2, CSPB1, PRKM14, PRKM15, SAPK2A, p38ALPHA, Hog, CRK1, CSBP, Exip, Csbp1, Csbp2, Prkm14, Prkm15, Sapk2A, p38Hog, p38alpha, MGC105413, p38b, zp38b, MAPK14, MGC142910, 186F5S, BG:DS00797.3, D-p38, D-p38b, Dm p38b, Dp38, ESTS:186F5S, Mpk34C, anon-sts23, p38 MAPK, p38 beta, p38B, p38beta, DmelCG7393, CG7393

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Protein Modifications, Cancer, Cell Cycle, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-MAPK14 Antibody (T180/Y182) (ABIN391334) in mouse lung tissue lysates (35ug/lane). MAPK14 (arrow) was detected using the purified Pab.

MAPK14 Antibody (T180/Y182)(ABIN391334) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MAPK14 Antibody (T180/Y182) for immunohistochemistry. Clinical relevance has not been evaluated.

Publications

Product: Dean, Sarsfield, Tsounakou et al.: "p38 Mitogen-activated protein kinase stabilizes mRNAs that contain cyclooxygenase-2 and tumor necrosis factor AU-rich elements by inhibiting deadenylation." in: The Journal of biological chemistry, Vol. 278, Issue 41, pp. 39470-6, 2003 (PubMed).

Cheung, Campbell, Nebreda et al.: "Feedback control of the protein kinase TAK1 by SAPK2a/p38alpha." in: The EMBO journal, Vol. 22, Issue 21, pp. 5793-805, 2003 (PubMed).