Calcium/calmodulin-Dependent Protein Kinase IG (CAMK1G) (Center, Lys226) antibody

Antigen: Calcium/calmodulin-Dependent Protein Kinase IG (CAMK1G)

Synonyms: VWS1, CLICKIII, dJ272L16.1, MGC30513, CLICK-III, CaMKIgamma, zgc:73127, wu:fk53c02, cam-k, CAMK1G, Ci-CaM-K, MGC142748, MGC73155, wu:fk61c03

Binding Site: Center, Lys226

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391377

Additional Information

Alternative name: CAMK1G

Gene ID: 57172

UniProt: NP_065172, Q96NX5

Immunogen: This CAMK1G antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 219~248 amino acids from the Center region of human CAMK1G.

Isotype: Ig

Description: Other names: calcium/calmodulin-dependent protein kinase IG

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This CAMK1G antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 219~248 amino acids from the Center region of human CAMK1G.

Molecular Weight: 53128 DA

Comments:

Background: Ca2+/calmodulin-dependent protein kinase I (CaMKI) constitutes a family of closely related isoforms (alpha, beta and gamma). CLICK-III/CaMKIgamma is a novel membrane-anchored neuronal Ca2+/calmodulin-dependent protein kinase. AMKIgamma is abundant in neurons, particularly in the amygdala and ventromedial hypothalamus. Like the other CaMKI isoforms, full activation of CLICK-III/CaMKIgamma requires both Ca(2+)/CaM and phosphorylation by CaMKK.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:50~100 Immunohistochemistry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure, Neurology

Restrictions: For Research Use only

Images

Western blot analysis of CAMK1G (Center K226)(ABIN391377) in K562 cell line lysates (35ug/lane). CAMK1G (arrow) was detected using the purified Pab.

Western blot analysis of CAMK1G (Center K226) (ABIN391377) in mouse cerebellum tissue lysates (35ug/lane). CAMK1G (arrow) was detected using the purified Pab.

Western blot analysis of CAMK1G (arrow) using rabbit polyclonal CAMK1G (Center K226) Antibody (ABIN391377). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with CAMK1G gene (Lane 2) (Origene Technologies).

Publications

Product: Takemoto-Kimura, Terai, Takamoto et al.: "Molecular cloning and characterization of CLICK-III/CaMKIgamma, a novel membrane-anchored neuronal Ca2+/calmodulin-dependent protein kinase (CaMK)." in: The Journal of biological chemistry, Vol. 278, Issue 20, pp. 18597-605, 2003 (PubMed).