RPTOR Independent Companion of MTOR, Complex 2 (RICTOR) (N-Term) antibody

Antigen: RPTOR Independent Companion of MTOR, Complex 2 (RICTOR)

Synonyms: AVO3, AW492497, 4921505C17Rik, 6030405M08Rik, D530039E11Rik, PIA, mAVO3, KIAA1999, MGC39830, DKFZp686B11164, Fyb, RICTOR

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391384

Additional Information

Alternative name: Rictor

Gene ID: 253260

UniProt: Q6R327

Immunogen: This Rictor antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 259~289 amino acids from the N-terminal region of human Rictor.

Isotype: Ig

Description: Other names: KIAA1999

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This Rictor antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 259~289 amino acids from the N-terminal region of human Rictor.

Molecular Weight: 192087 DA

Comments:

Background: RICTOR and MTOR (FRAP1) are components of a protein complex that integrates nutrient- and growth factor-derived signals to regulate cell growth.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blot~~1:50~100 Immunohistochemistry ~~ 1:50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Protein Modifications, Cell Cycle, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of Rictor Antibody (N-term) (ABIN391384) in Ramos cell line lysates (35ug/lane). RICTOR (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human Lymph tissue reacted with Rictor antibody (N-term) (ABIN391384), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Publications

Product: Jacinto, Facchinetti, Liu et al.: "SIN1/MIP1 maintains rictor-mTOR complex integrity and regulates Akt phosphorylation and substrate specificity." in: Cell, Vol. 127, Issue 1, pp. 125-37, 2006 (PubMed).