Golgi-Associated PDZ and Coiled-Coil Motif Containing (GOPC) (C-Term) antibody

Antigen: Golgi-Associated PDZ and Coiled-Coil Motif Containing (GOPC)

Synonyms: CAL, FIG, PIST, GOPC1, dJ94G16.2, fe38f01, MGC65853, zgc:56254, zgc:65853, wu:fe38f01, DKFZp459H2130

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391394

Additional Information

Alternative name: PIST

Gene ID: 57120

UniProt: Q9HD26

Immunogen: This PIST antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 368~398 amino acids from the C-terminal region of human PIST.

Isotype: Ig

Description: Other names: PDZ protein interacting specifically with TC10, CFTR-associated ligand, Fused in glioblastoma

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This PIST antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 368~398 amino acids from the C-terminal region of human PIST.

Molecular Weight: 50389 DA

Comments:

Background: PDZ domains contain approximately 90 amino acids and bind the extreme C terminus of proteins in a sequence-specific manner. PIST, a PDZ domain-containing Golgi protein, was discovered in a yeast two-hybrid system as a binding partner to Beclin-1, a Bcl-2-interacting protein homologous to the yeast autophagy gene apg6. Experiments with mutant PIST proteins lacking the PDZ domain showed that PIST interaction with Beclin-1 through its coiled-coil domain can modulate Beclin-1 activity and suggest that PIST interactions with other proteins through its PDZ domain may regulate the activity of PIST and Beclin-1.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Protein Modifications, Autophagy, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of PIST Antibody (C-term) (ABIN391394) in Ramos cell line lysates (35ug/lane). PIST (arrow) was detected using the purified Pab (1:60 dilution).

Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with PIST antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Publications

Product: Wente, Stroh, Beaudet et al.: "Interactions with PDZ domain proteins PIST/GOPC and PDZK1 regulate intracellular sorting of the somatostatin receptor subtype 5." in: The Journal of biological chemistry, Vol. 280, Issue 37, pp. 32419-25, 2005 (PubMed).