Mitogen-Activated Protein Kinase 4 (MAPK4) (C-Term) antibody

Antigen: Mitogen-Activated Protein Kinase 4 (MAPK4)

Synonyms: ERK3, Erk4, PRKM4, p63MAPK, Erk3, Prkm4, p63Mapk, A330097D03Rik, erk4, zgc:55498, wu:fi27d11

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391466

Additional Information

Alternative name: MAPK4

Gene ID: 5596

UniProt: Q0VG04

Immunogen: This MAPK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 544~574 amino acids from the C-term of human MAPK4.

Isotype: Ig

Description: Other names: Mitogen-activated protein kinase 4, Mitogen-activated protein kinase 4, isoform CRA_a, MAPK4

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This MAPK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 544~574 amino acids from the C-term of human MAPK4.

Molecular Weight: 65922 DA

Comments:

Background: Mitogen-activated protein kinase 4 is a member of the mitogen-activated protein kinase family. Tyrosine kinase growth factor receptors activate mitogen-activated protein kinases which then translocate into the nucleus where it phosphorylates nuclear targets.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Cycle, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-MAPK4 Antibody (C-term) (ABIN391466) in Jurkat cell line lysates (35ug/lane). MAPK4(arrow) was detected using the purified Pab (1:60 dilution).

Publications

Product: Seger, Krebs: "The MAPK signaling cascade." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 9, Issue 9, pp. 726-35, 1995 (PubMed).

Kant, Schumacher, Singh et al.: "Characterization of the atypical MAPK ERK4 and its activation of the MAPK-activated protein kinase MK5." in: The Journal of biological chemistry, Vol. 281, Issue 46, pp. 35511-9, 2006 (PubMed).

Lerner-Marmarosh, Miralem, Gibbs et al.: "Human biliverdin reductase is an ERK activator; hBVR is an ERK nuclear transporter and is required for MAPK signaling." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 19, pp. 6870-5, 2008 (PubMed).