|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Membrane Metallo-Endopeptidase (MME) (C-Term) antibody
|Synonyms||NEP, CD10, CALLA, MGC126681, MGC126707, DKFZp686O16152, C85356, 6030454K05Rik, MME|
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), ELISA
|4 references available|
|Quantity||0.1 mg (0.25 mg/ml)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||This Neprilysin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 513~542 amino acids from the C-terminal region of human Neprilysin.|
|Description||Other names: Neprilysin, Neutral endopeptidase 24.11, Neutral endopeptidase, NEP, Enkephalinase, Atriopeptidase, Common acute lymphocytic leukemia antigen, CALLA, CD_antigen=CD10, EPN, MME|
|Characteristics||Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This Neprilysin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 513~542 amino acids from the C-terminal region of human Neprilysin.|
|Molecular Weight||85514 DA|
Background: MME is a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). This protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. It is a oprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin.
|Application Notes||The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100 Flow cytometry ~~ 1:10~50|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Cell Structure|
|Restrictions||For Research Use only|
Shipp, Tarr, Chen et al.: "CD10/neutral endopeptidase 24.11 hydrolyzes bombesin-like peptides and regulates the growth of small cell carcinomas of the lung." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 23, pp. 10662-6, 1992 (PubMed).
Shipp, Vijayaraghavan, Schmidt et al.: "Common acute lymphoblastic leukemia antigen (CALLA) is active neutral endopeptidase 24.11 ("enkephalinase"): direct evidence by cDNA transfection analysis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, Issue 1, pp. 297-301, 1989 (PubMed).
Wang, Wang, Malter et al.: "Effects of HNE-modification induced by Abeta on neprilysin expression and activity in SH-SY5Y cells." in: Journal of neurochemistry, Vol. 108, Issue 4, pp. 1072-82, 2009 (PubMed).
Dakka, Bellaoui, Bouzid et al.: "CD10 AND CD34 expression in childhood acute lymphoblastic leukemia in Morocco: clinical relevance and outcome." in: Pediatric hematology and oncology, Vol. 26, Issue 4, pp. 216-31, 2009 (PubMed).