CK1 epsilon (CK1e) (C-Term) antibody

Antigen: CK1 epsilon (CK1e)

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391600

Additional Information

Gene ID: 1454

UniProt: P49674

Immunogen: This CK1e antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 338~368 amino acids from the C-terminal region of human CK1e.

Isotype: Ig

Description: Other names: Casein kinase I, epsilon isoform, CKI-epsilon, CKIe

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human CK1e.

Molecular Weight: 47315 DA

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-CK1e C-term Pab (ABIN391600) in SKBR3 cell lysate. CK1e (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Gao, Inuzuka, Tseng et al.: "Phosphorylation by Akt1 promotes cytoplasmic localization of Skp2 and impairs APCCdh1-mediated Skp2 destruction." in: Nature cell biology, Vol. 11, Issue 4, pp. 397-408, 2009 (PubMed).