ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 4 (ABCC4) (C-Term) antibody

Antigen: ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 4 (ABCC4)

Synonyms: MRP4, MOATB, MOAT-B, EST170205, Mrp4, D630049P08Rik

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391639

Additional Information

Alternative name: ABCC4

Gene ID: 10257

UniProt: O15439

Immunogen: This ABCC4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1124~1153 amino acids from the C-terminal region of human ABCC4.

Isotype: Ig

Description: Other names: Multi-specific organic anion transporter B, MOAT-B, MRP/cMOAT-related ABC transporter, ATP-binding cassette sub-family C member 4, Multidrug resistance-associated protein 4, ABCC4, MRP4

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This ABCC4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1124~1153 amino acids from the C-terminal region of human ABCC4.

Molecular Weight: 149527 DA

Comments:

Background: ABCC4 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC proteins are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. The specific function of this protein has not yet been determined, however, this protein may play a role in cellular detoxification as a pump for its substrate, organic anions.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-ABCC4 Antibody (C-term) (ABIN391639) in CEM cell line lysates (35ug/lane). ABCC4 (arrow) was detected using the purified Pab.

Publications

Product: Kool, de Haas, Scheffer et al.: "Analysis of expression of cMOAT (MRP2), MRP3, MRP4, and MRP5, homologues of the multidrug resistance-associated protein gene (MRP1), in human cancer cell lines." in: Cancer research, Vol. 57, Issue 16, pp. 3537-47, 1997 (PubMed).

Lee, Belinsky, Bell et al.: "Isolation of MOAT-B, a widely expressed multidrug resistance-associated protein/canalicular multispecific organic anion transporter-related transporter." in: Cancer research, Vol. 58, Issue 13, pp. 2741-7, 1998 (PubMed).

Adachi, Sampath, Lan et al.: "Expression of MRP4 confers resistance to ganciclovir and compromises bystander cell killing." in: The Journal of biological chemistry, Vol. 277, Issue 41, pp. 38998-9004, 2002 (PubMed).