DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 5 (DDX5) (Center) antibody

Antigen: DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 5 (DDX5)

Synonyms: p68, HLR1, G17P1, HUMP68, DKFZp434E109, DKFZp686J01190, Hlr1, MGC118083, 2600009A06Rik, wu:fa56a07, wu:fb11e01, wu:fb16c10, wu:fb53b05, ddx5, MGC76265, DDX5, DKFZp459M043

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391669

Additional Information

Alternative name: DDX5

Gene ID: 1655

UniProt: P17844

Immunogen: This DDX5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 313~342 amino acids from the Center region of human DDX5.

Isotype: Ig

Description: Other names: Probable ATP-dependent RNA helicase DDX5, DEAD box protein 5, RNA helicase p68 , HLR1, HELR, G17P1, DDX5

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This DDX5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 313~342 amino acids from the Center region of human DDX5.

Molecular Weight: 69148 DA

Comments:

Background: DDX5 is putative RNA helicases. The protein is implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This protein is a RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Translation Factors, Cancer, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of DDX5 antibody (Center) (ABIN391669) in Romas cell line lysates (35ug/lane). DDX5 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human brain with DDX5 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Publications

Product: Ong, Mittler, Mann: "Identifying and quantifying in vivo methylation sites by heavy methyl SILAC." in: Nature methods, Vol. 1, Issue 2, pp. 119-26, 2005 (PubMed).

Daub, Olsen, Bairlein et al.: "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." in: Molecular cell, Vol. 31, Issue 3, pp. 438-48, 2008 (PubMed).