Cyclin-Dependent Kinase 15 (ALS2CR7) (N-Term) antibody

Antigen: Cyclin-Dependent Kinase 15 (ALS2CR7)

Synonyms: PFTK2, ALS2CR7, als2cr7, MGC146368, MGC136819, zgc:136819, Pftk2, Als2cr7

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391735

Additional Information

Alternative name: ALS2CR7

Gene ID: 65061

UniProt: Q96Q40

Immunogen: This ALS2CR7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 6~35 amino acids from the N-terminal region of human ALS2CR7.

Isotype: Ig

Description: Other names: Serine/threonine-protein kinase ALS2CR7, Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This ALS2CR7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 6~35 amino acids from the N-terminal region of human ALS2CR7.

Molecular Weight: 43574 DA

Comments:

Background: Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure, Cell Cycle, Transcription Factors, Signaling, Metabolism

Restrictions: For Research Use only

Images

ALS2CR7 Antibody (R13) (ABIN391735) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the ALS2CR7 antibody detected the ALS2CR7 protein (arrow).

Publications

Product: Hadano, Hand, Osuga et al.: "A gene encoding a putative GTPase regulator is mutated in familial amyotrophic lateral sclerosis 2." in: Nature genetics, Vol. 29, Issue 2, pp. 166-73, 2001 (PubMed).