Cyclin-Dependent Kinase 10 (CDK10) (N-Term) antibody

Antigen: Cyclin-Dependent Kinase 10 (CDK10)

Synonyms: BC017131, zgc:110252, PISSLRE, MGC112847, MGC134436, cdc2rk, NV16107

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391741

Additional Information

Alternative name: CDK10

Gene ID: 8558

UniProt: Q15131

Immunogen: This CDK10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15~44 amino acids from the N-terminal region of human CDK10.

Isotype: Ig

Description: Other names: Cell division protein kinase 10, Serine/threonine-protein kinase PISSLRE

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This CDK10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15~44 amino acids from the N-terminal region of human CDK10.

Molecular Weight: 41038 DA

Comments:

Background: CDK10 belongs to the CDK subfamily of the Ser/Thr protein kinase family. The CDK subfamily members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and are known to be essential for cell cycle progression. This kinase has been shown to play a role in cellular proliferation. Its function is limited to cell cycle G2-M phase.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Cycle, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of CDK10 antibody (N-term) (ABIN391741) pre-incubated with (Lane1) and without (Lane 2) blocking peptide (BP7516a) in A375 cell line lysate. CDK10 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Leman, Magheli, Yong et al.: "Identification of nuclear structural protein alterations associated with seminomas." in: Journal of cellular biochemistry, Vol. 108, Issue 6, pp. 1274-9, 2009 (PubMed).

Product: Hershko, Ciechanover: "The ubiquitin system for protein degradation." in: Annual review of biochemistry, Vol. 61, pp. 761-807, 1992 (PubMed).

Brambilla, Draetta: "Molecular cloning of PISSLRE, a novel putative member of the cdk family of protein serine/threonine kinases." in: Oncogene, Vol. 9, Issue 10, pp. 3037-41, 1994 (PubMed).

Graña, Claudio, De Luca et al.: "PISSLRE, a human novel CDC2-related protein kinase." in: Oncogene, Vol. 9, Issue 7, pp. 2097-103, 1994 (PubMed).

Parge, Arvai, Murtari et al.: "Human CksHs2 atomic structure: a role for its hexameric assembly in cell cycle control." in: Science (New York, N.Y.), Vol. 262, Issue 5132, pp. 387-95, 1993 (PubMed).

Sherr: "Cancer cell cycles." in: Science (New York, N.Y.), Vol. 274, Issue 5293, pp. 1672-7, 1997 (PubMed).

Morgan: "Cyclin-dependent kinases: engines, clocks, and microprocessors." in: Annual review of cell and developmental biology, Vol. 13, pp. 261-91, 1998 (PubMed).

Crawford, Ianzano, Savino et al.: "The PISSLRE gene: structure, exon skipping, and exclusion as tumor suppressor in breast cancer." in: Genomics, Vol. 56, Issue 1, pp. 90-7, 1999 (PubMed).

Ganoth, Bornstein, Ko et al.: "The cell-cycle regulatory protein Cks1 is required for SCF(Skp2)-mediated ubiquitinylation of p27." in: Nature cell biology, Vol. 3, Issue 3, pp. 321-4, 2001 (PubMed).