|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Cyclin-Dependent Kinase 10 (CDK10) (N-Term) antibody
|Synonyms||BC017131, zgc:110252, PISSLRE, MGC112847, MGC134436, cdc2rk, NV16107|
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), ELISA
|9 references available|
|Quantity||0.1 mg (0.25 mg/ml)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||This CDK10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15~44 amino acids from the N-terminal region of human CDK10.|
|Description||Other names: Cell division protein kinase 10, Serine/threonine-protein kinase PISSLRE|
|Characteristics||Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This CDK10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15~44 amino acids from the N-terminal region of human CDK10.|
|Molecular Weight||41038 DA|
Background: CDK10 belongs to the CDK subfamily of the Ser/Thr protein kinase family. The CDK subfamily members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and are known to be essential for cell cycle progression. This kinase has been shown to play a role in cellular proliferation. Its function is limited to cell cycle G2-M phase.
|Application Notes||The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Cycle, Cell Structure|
|Restrictions||For Research Use only|
Leman, Magheli, Yong et al.: "Identification of nuclear structural protein alterations associated with seminomas." in: Journal of cellular biochemistry, Vol. 108, Issue 6, pp. 1274-9, 2009 (PubMed).
Hershko, Ciechanover: "The ubiquitin system for protein degradation." in: Annual review of biochemistry, Vol. 61, pp. 761-807, 1992 (PubMed).
Brambilla, Draetta: "Molecular cloning of PISSLRE, a novel putative member of the cdk family of protein serine/threonine kinases." in: Oncogene, Vol. 9, Issue 10, pp. 3037-41, 1994 (PubMed).
Graña, Claudio, De Luca et al.: "PISSLRE, a human novel CDC2-related protein kinase." in: Oncogene, Vol. 9, Issue 7, pp. 2097-103, 1994 (PubMed).
Parge, Arvai, Murtari et al.: "Human CksHs2 atomic structure: a role for its hexameric assembly in cell cycle control." in: Science (New York, N.Y.), Vol. 262, Issue 5132, pp. 387-95, 1993 (PubMed).
Sherr: "Cancer cell cycles." in: Science (New York, N.Y.), Vol. 274, Issue 5293, pp. 1672-7, 1997 (PubMed).
Morgan: "Cyclin-dependent kinases: engines, clocks, and microprocessors." in: Annual review of cell and developmental biology, Vol. 13, pp. 261-91, 1998 (PubMed).
Crawford, Ianzano, Savino et al.: "The PISSLRE gene: structure, exon skipping, and exclusion as tumor suppressor in breast cancer." in: Genomics, Vol. 56, Issue 1, pp. 90-7, 1999 (PubMed).
Ganoth, Bornstein, Ko et al.: "The cell-cycle regulatory protein Cks1 is required for SCF(Skp2)-mediated ubiquitinylation of p27." in: Nature cell biology, Vol. 3, Issue 3, pp. 321-4, 2001 (PubMed).
|Hosts||Rabbit (27), Goat (1)|
|Reactivities||Human (28), Rat (Rattus) (20), Mouse (Murine) (19), Chicken (18), Cow (Bovine) (18), Dog (Canine) (18), Horse (Equine) (18), Pig (Porcine) (18)|
|Applications||Immunofluorescence (IF) (15), Western Blotting (WB) (13), ELISA (11), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (5), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunohistochemistry (IHC) (2), Immunoelectron Microscopy (IEM) (1), Immunoprecipitation (IP) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||N-Term (2), Internal Region (1), N-Term,Arg5 (1)|