Homeodomain Interacting Protein Kinase 3 (HIPK3) (C-Term) antibody

Antigen: Homeodomain Interacting Protein Kinase 3 (HIPK3)

Synonyms: PKY, YAK1, DYRK6, FIST3, ANPK, HIPK3, pky, yak1, dyrk6, fist3, MGC145460

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391779

Additional Information

Alternative name: HIPK3

Gene ID: 10114

UniProt: Q9H422

Immunogen: This HIPK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1134~1163 amino acids from the sequence of human HIPK3 within a 1100-1200.

Isotype: Ig

Description: Other names: Homeodomain-interacting protein kinase 3, Homolog of protein kinase YAK1, Fas-interacting serine/threonine-protein kinase, FIST, Androgen receptor-interacting nuclear protein kinase, ANPK, DYRK6, FIST3, PKY

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This HIPK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1134~1163 amino acids from the sequence of human HIPK3 within a 1100-1200.

Molecular Weight: 133743 DA

Comments:

Background: HIPK3 negatively regulates apoptosis by promoting FADD phosphorylation. This kinase enhances androgen receptor-mediated transcription, and may act as a transcriptional corepressor for NK homeodomain transcription factors.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western Blot Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Apoptosis/Necrosis, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure, Cell Cycle, Transcription Factors, Signaling, Metabolism

Restrictions: For Research Use only

Images

Detection of interaction between HIPK3 and SF-1 by coimmunoprecipitation. After expression of SF-1-HA and Flag-sHIPK3 (aa 159 to 1191) in H1299 cells, the HIPK3 protein complex was immunoprecipitated with anti-Flag antibody or by direct loading to the gel (input). Western blotting was then performed to detect SF-1-HA and Flag-sHIPK3.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Tiefenbach, Moll, Nelson et al.: "A live zebrafish-based screening system for human nuclear receptor ligand and cofactor discovery." in: PLoS ONE, Vol. 5, Issue 3, pp. e9797, 2010 (PubMed).

Product: Blume-Jensen, Hunter: "Oncogenic kinase signalling." in: Nature, Vol. 411, Issue 6835, pp. 355-65, 2001 (PubMed).