EPH Receptor A5 (EPHA5) antibody

Antigen: EPH Receptor A5 (EPHA5)

Synonyms: CEK7, EHK1, HEK7, TYRO4, EPHA5

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391894

Additional Information

Alternative name: EphA5

Gene ID: 2044

UniProt: P54756

Immunogen: This EphA5 antibody is generated from rabbits immunized with his fusion recombinant protein of human EPHA5.

Isotype: Ig

Description: Other names: Ephrin type-A receptor 5, Tyrosine-protein kinase receptor EHK-1, Eph homology kinase-1, Receptor protein-tyrosine kinase HEK7

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This EphA5 antibody is generated from rabbits immunized with his fusion recombinant protein of human EPHA5.

Molecular Weight: 114784 DA

Comments:

Background: Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Stem Cells, Cell Signaling, Protein Modifications, Cell Cycle, Transcription Factors, Signaling, Metabolism, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of EphA5 (arrow) using rabbit polyclonal EphA5 Antibody (ABIN391894). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EphA5 gene (Lane 2) (Origene Technologies).

Publications

Product: Fox, Holst, Chute et al.: "cDNA cloning and tissue distribution of five human EPH-like receptor protein-tyrosine kinases." in: Oncogene, Vol. 10, Issue 5, pp. 897-905, 1995 (PubMed).