EPH Receptor B3 (EPHB3) (N-Term) antibody

Antigen: EPH Receptor B3 (EPHB3)

Synonyms: ETK2, HEK2, TYRO6, EK10, CEK10, EPHB3, tck, MGC83457

Epitope: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391922

Additional Information

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Alternative name: EphB3 (N-term)

Gene ID: 2049

Swiss-Prot: P54753

Immunogen: This EphB3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 37~66 amino acids from the N-terminal region of human EphB3.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: Ephrin type-B receptor 3, Tyrosine-protein kinase receptor HEK-2

Specificity: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human EphB3.

Molecular Weight: 110286 DA

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Stem Cells, Cell Signaling, Protein Modifications, Cell Structure, Neurology

Restrictions: For Research Use only

Images

Western blot analysis of anti-EphB3 N-term Pab (ABIN391922) in Jurkat cell lysate. EphB3 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.