Fibroblast Growth Factor Receptor 3 (FGFR3) (C-Term) antibody

Antigen: Fibroblast Growth Factor Receptor 3 (FGFR3)

Synonyms: ACH, CEK2, JTK4, CD333, HSFGFR3EX, sam3, HBGFR, Fgfr-3, Z-FGFR3, FGFR3, LOC100034114, ach, cek2, jtk4, cd333, hsfgfr3ex

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391969

Additional Information

Alternative name: FGFR3

Gene ID: 2261

UniProt: P22607

Immunogen: This FGFR3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 777~806 amino acids from the C-terminal region of human FGFR3.

Isotype: Ig

Description: Other names: fibroblast growth factor receptor 3

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This FGFR3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 777~806 amino acids from the C-terminal region of human FGFR3.

Molecular Weight: 87709 DA

Comments:

Background: FGFR3 is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein would consist of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member binds acidic and basic fibroblast growth hormone and plays a role in bone development and maintenance. Mutations in this gene lead to craniosynostosis and multiple types of skeletal dysplasia.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure, Cell Cycle

Restrictions: For Research Use only

Images

Western blot analysis of anti-FGFR3 Pab (ABIN391969) in Jurkat cell lysate. FGFR3 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Lievens, Liboi: "The thanatophoric dysplasia type II mutation hampers complete maturation of fibroblast growth factor receptor 3 (FGFR3), which activates signal transducer and activator of transcription 1 (STAT1) from the endoplasmic reticulum." in: The Journal of biological chemistry, Vol. 278, Issue 19, pp. 17344-9, 2003 (PubMed).

Sturla, Merrick, Burchill: "FGFR3IIIS: a novel soluble FGFR3 spliced variant that modulates growth is frequently expressed in tumour cells." in: British journal of cancer, Vol. 89, Issue 7, pp. 1276-84, 2003 (PubMed).