|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Macrophage Stimulating 1 Receptor (C-Met-Related tyrosine Kinase) (MST1R) antibody
|Synonyms||RON, PTK8, CD136, CDw136, Fv2, Ron, STK, Fv-2, Stk, Ptk8, Cdw136|
Alternatives Western Blotting (WB), ELISA
|3 references available|
|Quantity||0.1 mg (0.25 mg/ml) (Variants)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||This RON antibody is generated from rabbits immunized with two KLH conjugated synthetic peptides between 22~51 amino acids from the N-terminal and C-terminal regions of human RON.|
|Description||Other names: p185-Ron, CDW136, CD136 antigen, MST1R, RON, Macrophage-stimulating protein receptor alpha chain, Macrophage-stimulating protein receptor beta chain, Macrophage-stimulating protein receptor MSPR, MSP receptor|
|Characteristics||Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This RON antibody is generated from rabbits immunized with two KLH conjugated synthetic peptides between 22~51 amino acids from the N-terminal and C-terminal regions of human RON.|
|Molecular Weight||152227 DA|
Background: Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).
|Application Notes||The suggested dilution will be: ELISA~~1:1,000 Western blotting~~1:100~500|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Cycle, Transcription Factors, Signaling, Metabolism, Cell Structure|
|Restrictions||For Research Use only|
|Western blot analysis of anti-RON Pab (ABIN392048) in THP1 cell lysate. RON (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.|
Zhou, He, Chen et al.: "Altered expression of the RON receptor tyrosine kinase in primary human colorectal adenocarcinomas: generation of different splicing RON variants and their oncogenic potential." in: Oncogene, Vol. 22, Issue 2, pp. 186-97, 2003 (PubMed).
Danilkovitch-Miagkova, Duh, Kuzmin et al.: "Hyaluronidase 2 negatively regulates RON receptor tyrosine kinase and mediates transformation of epithelial cells by jaagsiekte sheep retrovirus." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 8, pp. 4580-5, 2003 (PubMed).
Penengo, Rubin, Yarden et al.: "c-Cbl is a critical modulator of the Ron tyrosine kinase receptor." in: Oncogene, Vol. 22, Issue 24, pp. 3669-79, 2003 (PubMed).
|Hosts||Rabbit (69), Mouse (5)|
|Reactivities||Human (70), Mouse (Murine) (49), Rat (Rattus) (47), Cat (Feline) (1), Chicken (1), Cow (Bovine) (1), Dog (Canine) (1)|
|Applications||Immunofluorescence (IF) (41), ELISA (21), Western Blotting (WB) (20), Flow Cytometry (FACS) (17), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (13), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (5), Immunohistochemistry (IHC) (5), Immunoprecipitation (IP) (4), Immunoelectron Microscopy (IEM) (2), Immunohistochemistry (Paraffin-embedded Sections) pro (IHC (p)+) (2), Immunocytochemistry (ICC) (1)|
|Conjugates||Alexa Fluor 350 (4), Alexa Fluor 488 (4), Alexa Fluor 555 (4), Alexa Fluor 647 (4), PE,Cy5.5 (4), Biotin (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy7 (2), Alexa Flour 488 (1)|
|Epitopes||pTyr1353 (17), pSer1349 (5), pTyr1238 (4), C-Term (3), Internal Region (2), N-Term (2), AA 210-320 (1), pSer1394 (1), pSer30 (1)|