anthrax Toxin Receptor 1 (ANTXR1) (Tyr382) antibody

Antigen: anthrax Toxin Receptor 1 (ANTXR1)

Synonyms: Tem8, Antrx1, 2310008J16Rik, 2810405N18Rik, ATR, TEM8, FLJ10601, FLJ11298, FLJ21776, ANTXR1

Binding Site: Tyr382

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN392154

Additional Information

Alternative name: ANTXR1

Gene ID: 84168

UniProt: Q9H6X2

Immunogen: This ANTXR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 365~394 amino acids surrounding Y382 of human ANTXR1.

Isotype: Ig

Description: Other names: Anthrax toxin receptor 1 [Precursor], Tumor endothelial marker 8, ANTXR1, ATR, TEM8

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This ANTXR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 365~394 amino acids surrounding Y382 of human ANTXR1.

Molecular Weight: 62789 DA

Comments:

Background: ANTXR1 is a type I transmembrane protein and is a tumor-specific endothelial marker that has been implicated in colorectal cancer. This protein has been shown to also be a docking protein or receptor for Bacillus anthracis toxin, the causative agent of the disease, anthrax. The binding of the protective antigen (PA) component, of the tripartite anthrax toxin, to this receptor protein mediates delivery of toxin components to the cytosol of cells. Once inside the cell, the other two components of anthrax toxin, edema factor (EF) and lethal factor (LF) disrupt normal cellular processes.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Signaling, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-ANTXR1 Antibody (Y382)(ABIN392154) in Hela cell line lysates (35ug/lane). ANTXR1 (arrow) was detected using the purified Pab (1:60dilution).

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ANTXR1 Antibody (Y382) (ABIN392154), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Publications

Product: Rainey, Wigelsworth, Ryan et al.: "Receptor-specific requirements for anthrax toxin delivery into cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 37, pp. 13278-83, 2005 (PubMed).

Wei, Lu, Chaudry et al.: "The LDL receptor-related protein LRP6 mediates internalization and lethality of anthrax toxin." in: Cell, Vol. 124, Issue 6, pp. 1141-54, 2006 (PubMed).

Werner, Kowalczyk, Faundez: "Anthrax toxin receptor 1/tumor endothelium marker 8 mediates cell spreading by coupling extracellular ligands to the actin cytoskeleton." in: The Journal of biological chemistry, Vol. 281, Issue 32, pp. 23227-36, 2006 (PubMed).