WW Domain Binding Protein 2 (WBP2) (C-Term) antibody

Antigen: WW Domain Binding Protein 2 (WBP2)

Synonyms: WBP-2, MGC18269, zgc:64061, wu:fc64g04, MGC68743, wbp-2

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN392214

Additional Information

Alternative name: WBP2

Gene ID: 23558

UniProt: Q969T9

Immunogen: This WBP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 192~221 amino acids from the C-terminal region of human WBP2.

Isotype: Ig

Description: Other names: WW domain-binding protein 2, WBP-2

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This WBP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 192~221 amino acids from the C-terminal region of human WBP2.

Molecular Weight: 28087 DA

Comments:

Background: The globular WW domain is composed of 38 to 40 semiconserved amino acids shared by proteins of diverse functions including structural, regulatory, and signaling proteins. The domain is involved in mediating protein-protein interactions through the binding of polyproline ligands. WBP2 is a WW domain binding protein, which binds to the WW domain of Yes kinase-associated protein by its PY motifs. The function of this protein has not been determined.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Signaling, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-BP2 Antibody (C-term) (ABIN392213) in A2058 cell line lysates (35ug/lane). BP2(arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human brain tissue reacted with WBP2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Flow cytometric analysis of HepG2 cells using WBP2 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Dhananjayan, Ramamoorthy, Khan et al.: "WW domain binding protein-2, an E6-associated protein interacting protein, acts as a coactivator of estrogen and progesterone receptors." in: Molecular endocrinology (Baltimore, Md.), Vol. 20, Issue 10, pp. 2343-54, 2006 (PubMed).