Receptor-Interacting serine-threonine Kinase 3 (RIPK3) (C-Term) antibody

Antigen: Receptor-Interacting serine-threonine Kinase 3 (RIPK3)

Synonyms: RIP3, Hcyp2, RIPK3, MGC152488

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN392275

Additional Information

Alternative name: RIPK3

Gene ID: 11035

UniProt: NP_006862

Immunogen: This RIPK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 489~518 amino acids from the C-terminal region of human RIPK3.

Isotype: Ig

Description: Other names: Receptor-interacting serine/threonine protein kinase 3, RIP-like protein kinase 3, Receptor-interacting protein 3, RIP-3, RIP3

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This RIPK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 489~518 amino acids from the C-terminal region of human RIPK3.

Molecular Weight: 56901 DA

Comments:

Background: RIPK3 is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, and contains a C-terminal domain unique from other RIP family members. The encoded protein is predominantly localized to the cytoplasm, and can undergo nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. It is a component of the tumor necrosis factor (TNF) receptor-I signaling complex, and can induce apoptosis and weakly activate the NF-kappaB transcription factor.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:50~100 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Apoptosis/Necrosis, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cancer, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of hRIPK3-E504 (ABIN392275) in K562 cell line lysates (35ug/lane). RIPK3 (arrow) was detected using the purified Pab.

Western blot analysis of hRIPK3-E504 (ABIN392275) in mouse cerebellum tissue lysates (35ug/lane). RIPK3 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with RIP3 (RIPK3) antibody (C-term) (ABIN392275), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Publications

Product: Sun, Lee, Navas et al.: "RIP3, a novel apoptosis-inducing kinase." in: The Journal of biological chemistry, Vol. 274, Issue 24, pp. 16871-5, 1999 (PubMed).