Testis-Specific Kinase 2 (TESK2) (Center,Cys225) antibody

Antigen: Testis-Specific Kinase 2 (TESK2)

Synonyms: DGS-G, SPOGA2, STK22B, FLJ38613, MGC29168, TESK2

Binding Site: Center,Cys225

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN392278

Additional Information

Alternative name: TESK2

Gene ID: 10420

UniProt: Q96S53

Immunogen: This TESK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 218~247 amino acids from the Center(C225) region of human TESK2.

Isotype: Ig

Description: Other names: Testicular protein kinase 2

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This TESK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 218~247 amino acids from the Center(C225) region of human TESK2.

Molecular Weight: 63509 DA

Comments:

Background: TESK2 is a dual specificity protein kinase activity catalyzing autophosphorylation and phosphorylation of exogenous substrates on both serine/threonine and tyrosine residues. TESK2 phosphorylates cofilin at Ser-3. It may play an important role in spermatogenesis. This nuclear enzyme is thought to be activated by autophosphorylation on Ser-219. Expression is predominantly in testis and prostate, with highest concentrations in nongerminal Sertoli cells.

Application Details

Application Notes: The suggested dilution is: ELISA1:1,000 Western blotting 1:50~100 Immunohistochemistry~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Apoptosis/Necrosis, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-TESK2 Antibody (Center) (ABIN392278) in HL60 cell line lysates (35ug/lane). TESK2 (arrow) was detected using the purified Pab (1:60 dilution).

Formalin-fixed and paraffin-embedded human testis tissue reacted with TESK2 antibody (Center)(ABIN392278), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Publications

Product: Røsok, Pedeutour, Ree et al.: "Identification and characterization of TESK2, a novel member of the LIMK/TESK family of protein kinases, predominantly expressed in testis." in: Genomics, Vol. 61, Issue 1, pp. 44-54, 1999 (PubMed).

Toshima, Toshima, Takeuchi et al.: "Cofilin phosphorylation and actin reorganization activities of testicular protein kinase 2 and its predominant expression in testicular Sertoli cells." in: The Journal of biological chemistry, Vol. 276, Issue 33, pp. 31449-58, 2001 (PubMed).