Butyrylcholinesterase (BCHE) (Center) antibody

Antigen: Butyrylcholinesterase (BCHE)

Synonyms: E1, CHE1, che, che1, bche

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN392290

Additional Information

Alternative name: BCHE

Gene ID: 590

UniProt: P06276

Immunogen: This BCHE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 393~422 amino acids from the center region of human BCHE.

Isotype: Ig

Description: Other names: Pseudocholinesterase, Butyrylcholine esterase, Choline esterase II, Acylcholine acylhydrolase, Cholinesterase, BCHE, CHE1

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This BCHE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 393~422 amino acids from the center region of human BCHE.

Molecular Weight: 68418 DA

Comments:

Background: Mutant proteins of BCHE are responsible for suxamethonium sensitivity. Homozygous persons sustain prolonged apnea after administration of the muscle relaxant suxamethonium in connection with surgical anesthesia. The activity of pseudocholinesterase in the serum is low and its substrate behavior is atypical. In the absence of the relaxant, the homozygote is at no known disadvantage.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Metabolism, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-BCHE Antibody (Center)(ABIN392290) in A2058 cell line lysates (35ug/lane). BCHE (arrow) was detected using the purified Pab.

Publications

Product: Primo-Parmo, Bartels, Wiersema et al.: "Characterization of 12 silent alleles of the human butyrylcholinesterase (BCHE) gene." in: American journal of human genetics, Vol. 58, Issue 1, pp. 52-64, 1996 (PubMed).