GAPDH antibody (N-Term)

Catalog No. ABIN392353

This Rabbit Polyclonal antibody specifically detects GAPDH in WB, IF and IHC (p). It exhibits reactivity toward Human. It has been mentioned in 14+ publications

Quick Overview for GAPDH antibody (N-Term) (ABIN392353)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GAPDH antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB16537

Product Details anti-GAPDH Antibody

Binding Specificity: AA 62-91, N-Term

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This GAPDH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-91 amino acids from the N-terminal region of human GAPDH.

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:10~50. IF: 1:10~50. WB: 1:1000. WB: 1:500. WB: 1:1000. IHC-P: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-GAPDH antibody (ABIN392353)

Rao, Xia, Yang, Hu, Hu, Lei, Wu, Zhu: "Transient scrotal hyperthermia affects human sperm DNA integrity, sperm apoptosis, and sperm protein expression." in: Andrology, Vol. 4, Issue 6, pp. 1054-1063, (2017) (PubMed).

Shi, Qu, Li, Sui, Yao, Yang, Shi, Ji, Hou: "Increased expression of EHF via gene amplification contributes to the activation of HER family signaling and associates with poor survival in gastric cancer." in: Cell death & disease, Vol. 7, Issue 10, pp. e2442, (2017) (PubMed).

Yu, Lu, Han, Zhao, Li, Mao, Wang, Shen, Fan, Wang, Wang, Li, Tang, Song: "microRNA -140-5p inhibits colorectal cancer invasion and metastasis by targeting ADAMTS5 and IGFBP5." in: Stem cell research & therapy, Vol. 7, Issue 1, pp. 180, (2017) (PubMed).

Qiang, Sui, Ma, Li, Ren, Shao, Liu, Guan, Shi, Hou: "Proteasome inhibitor MG132 induces thyroid cancer cell apoptosis by modulating the activity of transcription factor FOXO3a." in: Endocrine, Vol. 56, Issue 1, pp. 98-108, (2017) (PubMed).

Zhou, Liu, Li, Zhang, Wang, Lan, Shi, Li, Ju: "Migration ability and Toll-like receptor expression of human mesenchymal stem cells improves significantly after three-dimensional culture." in: Biochemical and biophysical research communications, Vol. 491, Issue 2, pp. 323-328, (2017) (PubMed).

Ho, Morrison, Silva, Coomber: "The effect of 3-bromopyruvate on human colorectal cancer cells is dependent on glucose concentration but not hexokinase II expression." in: Bioscience reports, Vol. 36, Issue 1, pp. e00299, (2016) (PubMed).

Xu, Han, Tang, Zeng, Lin, Zhao, Zhang, Cao, Wu, Mao: "The Ring Finger Protein RNF6 Induces Leukemia Cell Proliferation as a Direct Target of Pre-B-cell Leukemia Homeobox 1." in: The Journal of biological chemistry, Vol. 291, Issue 18, pp. 9617-28, (2016) (PubMed).

Li, Lu, Wang, Han, Mao, Li, Yu, Wang, Fan, Yu, Song: "miR-221/222 enhance the tumorigenicity of human breast cancer stem cells via modulation of PTEN/Akt pathway." in: Biomedicine & pharmacotherapy, Vol. 79, pp. 93-101, (2016) (PubMed).

Wan, Qin, Zhang, Lu, Zhu, Zhang, Dai, Shi, Ye: "Oxidized low-density lipoprotein is associated with advanced-stage prostate cancer." in: Tumour biology, Vol. 36, Issue 5, pp. 3573-82, (2015) (PubMed).

Wang, Cui, Wang, Xue, Chen, Yu, Lu, Zhang, Tang, Peng: "Elevated kinesin family member 26B is a prognostic biomarker and a potential therapeutic target for colorectal cancer." in: Journal of experimental & clinical cancer research : CR, Vol. 34, pp. 13, (2015) (PubMed).

Li, Chen, Shi, Li, Cai, Zhou, Zhong, Yao: "Angiopoietin-like 4 enhances metastasis and inhibits apoptosis via inducing bone morphogenetic protein 7 in colorectal cancer cells." in: Biochemical and biophysical research communications, Vol. 467, Issue 1, pp. 128-34, (2015) (PubMed).

Zhu, Zhang, Li, Huang, Sun, Fu, Guan: "Prognostic significance of FAM3C in esophageal squamous cell carcinoma." in: Diagnostic pathology, Vol. 10, Issue 1, pp. 192, (2015) (PubMed).

He, Mangala, Theriot, Rohde, Wu, Zhang: "Cell killing and radiosensitizing effects of atorvastatin in PC3 prostate cancer cells." in: Journal of radiation research, Vol. 53, Issue 2, pp. 225-33, (2012) (PubMed).

Yang, Wang, Yu, Wang, Yuan, Zhang: "MicroRNAs are involved in erythroid differentiation control." in: Journal of cellular biochemistry, Vol. 107, Issue 3, pp. 548-56, (2009) (PubMed).

Target Details for GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name: GAPDH

Background: GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains.

Molecular Weight: 36053

Gene ID: 2597

NCBI Accession: NP_001243728, NP_002037

UniProt: P04406