U2AF Homology Motif (UHM) Kinase 1 (UHMK1) (N-Term) antibody

Antigen: U2AF Homology Motif (UHM) Kinase 1 (UHMK1)

Synonyms: KIS, Kist, AA673513, AI449218, AU021979, 4732477C12Rik, 4930500M09Rik, KIST, MGC112620, UHMK1, MGC153241, zgc:153241, DKFZp469M0727

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN392641

Additional Information

Alternative name: KIST (KIS)

Gene ID: 127933

UniProt: Q8TAS1

Immunogen: This KIS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of human KIS.

Isotype: Ig

Description: Other names: Serine/threonine-protein kinase Kist, Kinase interacting with stathmin, U2AF homology motif kinase 1, UHMK1

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This KIS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of human KIS.

Molecular Weight: 46546 DA

Comments:

Background: Upon serum stimulation, KIS, a member of the Ser/Thr protein kinase family, phosphorylates CDKN1B/p27Kip1, thus controlling CDKN1B subcellular location and cell cycle progression in G1 phase. This protein, which contains 1 RRM (RNA recognition motif)domain, may be involved in trafficking and/or processing of RNA. KIS is widely expressed, with highest levels in skeletal muscle, kidney, placenta and peripheral blood leukocytes.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Cycle, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-KIS Pab (ABIN392641) in mouse heart tissue lysate. KIS (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

KIS Antibody (C6) (ABIN392641) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the KIS antibody detected the KIS protein (arrow).

Publications

Product: Boehm, Yoshimoto, Crook et al.: "A growth factor-dependent nuclear kinase phosphorylates p27(Kip1) and regulates cell cycle progression." in: The EMBO journal, Vol. 21, Issue 13, pp. 3390-401, 2002 (PubMed).

Bièche, Manceau, Curmi et al.: "Quantitative RT-PCR reveals a ubiquitous but preferentially neural expression of the KIS gene in rat and human." in: Brain research. Molecular brain research, Vol. 114, Issue 1, pp. 55-64, 2003 (PubMed).