NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2) (Center) antibody

Antigen: NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2)

Synonyms: NLK1, NEK2A, HsPK21

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN392649

Additional Information

Alternative name: NEK2

Gene ID: 4751

UniProt: Q96QN9

Immunogen: This NEK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 359~388 amino acids from the center region of human NEK2.

Isotype: Ig

Description: Other names: Serine threonine protein kinase Nek2, HsPK 21, HsPK21, NEK 2, NEK 2A, NEK2A, Never in mitosis gene A related kinase 2, NIMA (never in mitosis gene a) related kinase 2, NimA like protein kinase 1, NIMA related kinase 2, NimA related protein kinase 2, NLK 1

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This NEK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 359~388 amino acids from the center region of human NEK2.

Molecular Weight: 44905 DA

Comments:

Background: NEK2 is closely related in its catalytic domain to the serine/threonine protein kinase NIMA of Aspergillus nidulans which is required for entry into mitosis and may function in parallel to the universal mitotic inducer p34cdc2. Like NIMA, the Nek2 protein is almost undetectable during G1 but accumulates progressively throughout S, reaching maximal levels in late G2. These observations demonstrate that NEK2 resembles Aspergillus NIMA, both in its catalytic domain, and cell cycle-dependent expression. Recombinant NEK2 is active as a serine/threonine-specific protein kinase and may undergo autophosphorylation. Both human NEK2 and fungal NIMA phosphorylate a similar, but not identical, set of proteins and synthetic peptides. NEK2 is shown to be expressed most abundantly in adult testis and there is increasing evidence that NEK2 is abnormally expressed in a wide variety of human cancers. This antibody is specific to isoform 2 of NEK2.

Application Details

Application Notes: The suggested dilution will be: ELISA~~1:1,000 Western blotting~~1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure, Cell Cycle, Transcription Factors, Signaling, Metabolism

Restrictions: For Research Use only

Images

The anti-NEK2 Pab (ABIN392649) is used in Western blot to detect NEK2 in HeLa cell lysate.

Publications

Product: Blume-Jensen, Hunter: "Oncogenic kinase signalling." in: Nature, Vol. 411, Issue 6835, pp. 355-65, 2001 (PubMed).