LGR4 antibody (Internal Region)

Catalog No. ABIN400822

This Mouse Monoclonal antibody specifically detects LGR4 in WB, IHC (p) and EIA. It exhibits reactivity toward Human, Chimpanzee, Macaque and Orang-Utan.

Quick Overview for LGR4 antibody (Internal Region) (ABIN400822)

Target: LGR4 (Leucine-Rich Repeat Containing G Protein-Coupled Receptor 4 (LGR4))

Reactivity: Human, Chimpanzee, Macaque, Orang-Utan

Host: Mouse

Clonality: Monoclonal

Conjugate: This LGR4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Clone: 6G8-B3-G5-C3

Product Details anti-LGR4 Antibody

Binding Specificity: Internal Region

Specificity: This antibody is specific for Human LGR4 protein.

Cross-Reactivity (Details): Species reactivity (expected):Chimpanzee, Orangutan and Macaque (100 %).
Species reactivity (tested):Human.

Characteristics: Synonyms: G-protein coupled receptor 48, Leucine-rich repeat-containing G-protein coupled receptor4

Purification: Protein A Chromatography.

Immunogen: Synthetic peptide corresponding to an internal region of Human LGR4 protein

Isotype: IgG2b

Application Details

Application Notes: ELISA: 1/20,000. Western blot: 1/500-1/3,000 (Expect a band approximately 102 kDa in size correspondingto LGR4 protein in the appropriate cell lysate or extract). Immunohistochemistry on Paraffin Sections: 5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.13 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C to -70 °C forlonger. Avoid repeated freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for LGR4

Target: LGR4 (Leucine-Rich Repeat Containing G Protein-Coupled Receptor 4 (LGR4))

Alternative Name: LGR4 / GPR48

Background: LGR4, also known as leucine-rich repeat-containing G protein-coupled receptor 4, is a G protein-coupled receptors (GPCRs). GPCRs are membrane bound proteins that play key roles in a variety of physiologic functions. Members of the leucine-rich GPCR (LGR) family, such as GPR48, have multiple N-terminal leucinerich repeats (LRRs) and a 7-transmembrane domain. LGR4 is an orphan GPCR reported to be expressed in steroidogenic tissues such as placenta, ovary, testis, adrenal, pancreas, prostate, and thyroid, as well as in spinal cord, stomach, heart, and kidney.Synonyms: G-protein coupled receptor 48, Leucine-rich repeat-containing G-protein coupled receptor 4

Gene ID: 55366

UniProt: Q9BXB1