ASAP3 antibody

Catalog No. ABIN401043

The Rabbit Polyclonal anti-ASAP3 antibody (ABIN401043) specifically detects ASAP3 in WB and EIA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for ASAP3 antibody (ABIN401043)

Target: ASAP3 (ArfGAP with SH3 Domain, Ankyrin Repeat and PH Domain 3 (ASAP3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ASAP3 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-ASAP3 Antibody

Specificity: This antibody is directed against UPLC1/ASAP3 protein.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography on Protein A

Immunogen: Recombinant human UPLC1/ASAP3 protein

Isotype: IgG

Application Details

Application Notes: ELISA: 1/1,000 - 1/5,000. Western Blot: 1/1000 - 1/10,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.10 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for ASAP3

Target: ASAP3 (ArfGAP with SH3 Domain, Ankyrin Repeat and PH Domain 3 (ASAP3))

Alternative Name: DDEFL1

Background: UPLC1 (up-regulated in liver cancer 1), also named DDEFL1 (development and differentiation-enhancing factor-like 1) or ASAP3, is a member of the AZAP family of proteins. These proteins catalyze the hydrolysis of GTP bound to ADP-ribosylation factor (Arf) proteins, thereby causing Arf inactivation. For this reason, the ASAPs are generally called ArfGAPs. The activity of ArfGAPs is dependent on the presence of phosphoinositides and is implicated in cellular processes such as membrane trafficking and remodeling of the actin cytoskeleton. Both ASAP1 and ASAP3 have recently been implicated in the development of human cancers. Abnormal activity of ArfGAPs may lead to the alterations characteristic of malignant cells, such as the high rate of proliferation and invasion. More specifically, ASAP3 has been found to be up-regulated in 80 % of the hepatocellular carcinomas examined. Initial biochemical characterization reveals that ASAP3 shows class-specific GAP activity on Arf proteins, preferring Arf5 over Arf1 and Arf6.Synonyms: ACAP4, ANK repeat and PH domain-containing protein 3, ASAP3, Arf-GAP with SH3 domain, CENTB6, Development and differentiation-enhancing factor-like 1, Protein up-regulated in liver cancer 1, UPLC1

Gene ID: 55616

NCBI Accession: NP_001137250

UniProt: Q8TDY4