Protein G antibody (HRP)

Catalog No. ABIN401071

This Rabbit Polyclonal antibody specifically detects Protein G in DB, WB, EIA and IHC (fro). It exhibits reactivity toward Streptococcus.

Quick Overview for Protein G antibody (HRP) (ABIN401071)

Target: Protein G

Reactivity: Streptococcus

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Protein G antibody is conjugated to HRP

Application: Dot Blot (DB), Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Protein G Antibody (HRP)

Purification: Ion exchange chromatography

Immunogen: Protein G [Streptococcus species]

Isotype: IgG

Application Details

Application Notes: Western Blot. Dot Blot. ELISA: 1/20000 to 1/100000. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 2.0 mL of deionized water (or equivalent). For extended storage reconstitute product with 50 % glycerol.

Concentration: 10.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/mL Bovine Serum Albumin (BSA) as stabilizer and 0.01 % (w/v) Gentamicin Sulfate as preservative

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for Protein G

Target: Protein G

Background: Protein G is a bacterial protein derived from the cell wall of certain strains of b-hemolytic Streptococcci. It binds with high affinity to the Fc portion of various classes and subclasses of immunoglobulins from a variety of species. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse. Protein G binds preferentially to the Fc portion of IgG, but unlike Protein A can also bind to the Fab region, making it useful for purification of F(ab') fragments of IgG. Due to it's affinity for the Fc region of many mammalian immunoglobulins, protein G is considered a universal reagent in biochemistry and immunology.