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Protein G antibody (Biotin)

Reactivity: Streptococcus ELISA Host: Rabbit Polyclonal Biotin
Catalog No. ABIN5596972
  • Target See all Protein G Antibodies
    Protein G
    Reactivity
    Streptococcus
    Host
    • 8
    • 1
    Rabbit
    Clonality
    • 9
    Polyclonal
    Conjugate
    • 3
    • 2
    • 2
    • 1
    • 1
    This Protein G antibody is conjugated to Biotin
    Application
    • 6
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA
    Cross-Reactivity (Details)
    Cross reactivity against Protein G from other sources may occur but have not been specifically determined.
    Purification
    Anti-Protein G is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Protein G [Streptococcus species].
    Immunogen

    Immunogen: Protein G [Streptococcus species]

    Immunogen Type: Native Protein

    Isotype
    IgG
    Top Product
    Discover our top product Protein G Primary Antibody
  • Application Notes

    Application Note: Anti-Protein G Biotin Conjugated Antibody has been assayed against 1.0 μg of Protein G in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:90,000 to 1:350,000 of the reconstitution concentration is suggested for this product.

    ELISA Dilution: 1:90,000-1:350,000

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Expiry Date
    12 months
  • Target
    Protein G
    Abstract
    Protein G Products
    Background

    Synonyms: BAC, Protein G Biotin, Anti-Protein G BAC Antibody

    Background: Biotin is widely used throughout the biotechnology industry to conjugate proteins for biochemical assays. Biotin's small size typically does not affect the biological activity of protein upon biotinylation. Biotinylated proteins of interest can be enriched from a sample due to highly stable interactions. Biotin conjugated anti-Protein G antibodies are used as an amplifying reagent in immunohistochemistry, microarray assays, ELISAs, blots, and other applications.

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