GM-CSF antibody
| Antigen |
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| Synonyms |
GMCSF, GM-CSF |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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 Alternatives Enzyme Immunoassay (EIA), Neutralization (Neut), Western Blotting (WB)
Western Blotting (WB) (72), Enzyme Immunoassay (EIA) (53), Flow Cytometry (FACS) (42), Immunofluorescence (IF) (40), ELISA (34), Functional Studies (Func) (8), Immunohistochemistry (Frozen Sections) (IHC (fro)) (8), Immunoprecipitation (IP) (6), ELISA (Detection) (5), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (5), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Blocking Antibody (Inhibition) (3), ELISPOT (3), Neutralization (Neut) (3), Immunoelectron Microscopy (IEM) (2), Radioimmunoassay (RIA) (2), ELISA (Capture) (1), Immunohistochemistry (IHC) (1)
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| Catalog no. |
ABIN401109 |
| Quantity |
0.1 mg (1.0 mg/ml (by UV absorbance at 280 ...) (Variants) |
| Price |
310.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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Synonyms: CSF2, GMCSF, Sargramostim, Molgramostin, Granulocyte-macrophage colony-stimulatingfactor, Colony stimulating factor 2
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Swiss-Prot
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P01587
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Immunogen
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This purified antibody was prepared from whole rabbit serum produced by repeatedimmunizations with full length recombinant mouse GM-CSF protein.
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Cross-Reactivity
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Mouse (Murine)
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Isotype
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IgG (Matching secondary antibodies)
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Description
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Granulocyte Macrophage Colony Stimulating Factor (also known as GM-CSF,Colony-stimulating factor, CSF, sargramostim and molgramostin) is produced in responseto a number of inflammatory mediators by mesenchymal cells present in the hemopoieticenvironment and at peripheral sites of inflammation. Granulocyte Macrophage-CSF is ableto stimulate the production of neutrophilic granulocytes, macrophages, and mixedgranulocyte-macrophage colonies from bone marrow cells and can stimulate the formationof eosinophil colonies from fetal liver progenitor cells. GM-CSF can also stimulate somefunctional activities in mature granulocytes and macrophages. GM-CSF receptors showsignificant homologies with other receptors for hematopoietic growth factors, includingIL2-beta, IL-3, IL-6, IL-7, EPO and the Prolactin receptors.
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Specificity
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This antibody reacts to Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF). Species: Mouse. Others not tested.
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Application Notes
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ELISA: 1/20000 - 1/100000. Western Blot: 1/500 - 1/2000. Neutralization: 1/400. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Concentration
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1.0 mg/ml (by UV absorbance at 280 nm)
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Purification
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Purified
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Buffer
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0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2Reconstitution: Restore with 0.1 mL of deionized water (or equivalent).
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Storage
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Prior to reconstitution store at 2-8°C. Following reconstitution store the antibody undiluted at 2-8°C for one monthor (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
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Research Area
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Immunology, Innate Immunity, Cytokines
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Restrictions
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For Research Use only
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Alternatives for antigen "GM-CSF", type "Antibodies"
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Hosts
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Rabbit (70), Mouse (42), Rat (27), Sheep (1)
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Reactivities
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Human (84), Mouse (Murine) (48), Rat (Rattus) (28), Cow (Bovine) (19), Dog (Canine) (17), Chicken (16), Pig (Porcine) (16), Sheep (Ovine) (3), Monkey (1), Rabbit (1)
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Applications
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Western Blotting (WB) (72), Enzyme Immunoassay (EIA) (53), Flow Cytometry (FACS) (42), Immunofluorescence (IF) (40), ELISA (34), Functional Studies (Func) (8), Immunohistochemistry (Frozen Sections) (IHC (fro)) (8), Immunoprecipitation (IP) (6), ELISA (Detection) (5), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (5), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Blocking Antibody (Inhibition) (3), ELISPOT (3), Neutralization (Neut) (3), Immunoelectron Microscopy (IEM) (2), Radioimmunoassay (RIA) (2), ELISA (Capture) (1), Immunohistochemistry (IHC) (1)
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Conjugates
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Biotin (15), HRP (3), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2)
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