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Interleukin 27 (IL27) antibody (HRP)

Details for Product No. ABIN401111
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Antigen
Synonyms IL27, IL-17D, IL-22, IL-27, IL-27A, IL27A, IL27p28, IL30, p28, IL-27p28, Il30, RGD1561420
Reactivity
Mouse (Murine)
(40), (32), (2), (1), (1)
Host
Rabbit
(47), (15), (5), (3)
Clonality
Polyclonal
Conjugate
HRP
(4), (3), (3), (2), (2), (2)
Application
ELISA, Western Blotting (WB)
(59), (29), (8), (6), (6), (4), (4), (4), (3), (3), (1), (1), (1)
Pubmed 1 reference available
Quantity 0.1 mg
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Catalog No. ABIN401111
390.50 $
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Immunogen Full length recombinant mouse IL27/p28 protein
Isotype IgG
Specificity This antibody reacts to Interleukin-27A.
Purification Ion exchange chromatography
Alternative Name Interleukin-27 / IL27A
Background The cytokine Interleukin 27 (IL-27) is produced in response to inflammation. It is made by activated antigen presenting cells including monocytes, endothelial cells, and dendritic cells. IL-27 consists of a heterodimeric combination of Epstein-Barr virus-induced molecule 3 (EBI3, or IL-27B) non-covalently linked with IL-27 p28 (or IL-27A). It is a regulator of T helper cell development and suppressor of T-cell proliferation. IL-27 has both pro- and anti- inflammatory properties. It can stimulate cytotoxic T cell activity and induce isotype switching in B-cells. It has diverse effects on innate immune cells. It induces monocytes and mast cells to secrete pro-inflammatory cytokines. When infection is present, IL-27 induces naive CD4+ T cells to proliferate and develop Th1 cell responses. As an anti- inflammatory regulator, IL-27 can inhibit Th1 or Th2 responses and restrict the strength and duration of adaptive immune responses. The IL-27 p28 subunit, a 28 kDa glycoprotein belonging to the type I cytokine family, is homologous to IL-12 p35, IL-23 p19, and IL-6. The EBI3 (Epstein-Barr virus-induced molecule 3, or IL-27B) subunit is a 34 kDa glycoprotein containing two fibronectin type III domains, and belongs to the type I cytokine receptor family. It can exist as a homodimer and can also heterodimerize with IL-12 p35. It is homologous to the p40 subunit of IL-12 and IL-23 and to the extracellular domain of IL-6 R. EBI3 can heterodimerize also with IL-12 p35, or can exist as a homodimer. The heterodimeric IL-27 receptor contains WSX-1 (TCCR) and gp130. WSX-1 is specific for IL-27, and is expressed on resting/naive CD4+ T cells, CD8+ T cells, NK cells, dendritic cells, monocytes, mast cells, and B cells. Gp130, on the other hand, functions as a subunit of the receptor complexes for at least seven other cytokines. IL-27 also promotes effector functions of NK cells and CD8+ T cells.
Alternate names: IL-27 subunit alpha, IL-30, IL27-A, IL30, Interleukin-30, p28
Gene ID 246779
NCBI Accession NP_663611.1
UniProt Q8K3I6
Application Notes ELISA: 1/10,000 - 1/50,000. Western Blot: 1/1,000 - 1/5,000. Immunohistochemistry: 1/500 - 1/2,500. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Restore with 0.1 mL of deionized water (or equivalent).
Concentration 1.0 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml Bovine Serum Albumin as stabilizer and 0.01% (w/v) Gentamicin Sulfate as preservative(Do NOT add Sodium Azide!)
Preservative Gentamicin sulfate
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Prior to reconstitution store at 2-8°C. Following reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at-20°C for longer.
Expiry Date 12 months
Background publications Pflanz, Timans, Cheung et al.: "IL-27, a heterodimeric cytokine composed of EBI3 and p28 protein, induces proliferation of naive CD4(+) T cells." in: Immunity, Vol. 16, Issue 6, pp. 779-90, 2002 (PubMed).

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