Phycoerythrin, R- antibody (Biotin)

Antigen: Phycoerythrin, R-

Clonality: Polyclonal

Host: Goat

Conjugate: Biotin

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Dot Blot (Dot), Enzyme Immunoassay (EIA), Western Blotting (WB)

Catalog no.: ABIN401158

Additional Information

Characteristics: Synonyms: rpeB

Alternative name: R-Phycoerythrin

Swiss-Prot: Q7SIF9

Immunogen: Highly purified R-Phycoerythrin from the seaweed gracila

Isotype: IgG

Description: Phycoerythrin is one of a series of fluorescent pigments known as phycobiliproteins, whichare produced by red and blue green algae. It occurs in more than one form, and has foundapplication in immunology and diagnostic medicine. B and R Phycoerythrins providesuperior labeling compared to fluorescein and rhodamine, and are used for labelingantibodies, usually monoclonals. These dyes may also be coupled to enzymes and otherproteins, nucleic acids, polypeptide hormones, drugs, etc. Since phycoerythrins absorblight maximally between 450 and 650nm they fill the need for an intense fluorescent dye inthe longer wavelengths of the visible spectrum, thereby avoiding interference fromnaturally fluorescing biological substances. R Phycoerythrin (240 kDa) is a labile moleculethat may dissociate into components upon exposure to reducing or denaturing agents.

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-GoatSerum and R-Phycoerythrin conjugated IgG. This antibody will cross react with B-Phycoerythrin. Reactivity with other phycobiliproteinsis unknown.

Application Details

Application Notes: Suitable for Immunoblotting (western or dot blot), ELISA as well as other Immunochemicalassays. R-Phycoerythrin (240 kDa) is a labile molecule that may dissociate intocomponents upon exposure to reducing or denaturing agents. Reaction with low molecularweight fragments is typically noted by western blot. Recommneded Dilutions: ELISA: 1/200,000. Western Blot: 1/2000-1/20,000. Immunohistochemistry: 1/500-1/3,000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)

Buffer: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 containing 10mg/ml BSA as stabilizer and 0.01% (w/v) Sodium Azide as preservativeLabel: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Molar Ratio: 10-20 BAC molecules per Goat IgG moleculeRe

Storage: Prior to reconstitution store at 2-8°C. Following reconstitution store the antibody undiluted at 2-8°C for one monthor (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only