AP2A2 antibody (AA 3-14)

Catalog No. ABIN401165

The Goat Polyclonal anti-AP2A2 antibody is suitable to detect AP2A2 in samples from Human, Mouse, Rat, Dog, Chicken, Chimpanzee and Orang-Utan. It has been validated for WB and EIA.

Quick Overview for AP2A2 antibody (AA 3-14) (ABIN401165)

Target: AP2A2 (Adaptor-Related Protein Complex 2, alpha 2 Subunit (AP2A2))

Reactivity: Human, Mouse, Rat, Dog, Chicken, Chimpanzee, Orang-Utan

Host: Goat

Clonality: Polyclonal

Conjugate: This AP2A2 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-AP2A2 Antibody

Binding Specificity: AA 3-14

Specificity: This antibody is directed against AP2A.

Cross-Reactivity (Details): Species reactivity (expected):Chimpanzee, Orangutan, Mouse, Rat, Chicken, Canine.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Synthetic peptide corresponding aa 3-14 of Human AP2A1 and AP2A2 proteins

Isotype: IgG

Application Details

Application Notes: ELISA: 1/5,000 - 1/20,000. Western blot: 1/500 - 1/2,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.7 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for AP2A2

Target: AP2A2 (Adaptor-Related Protein Complex 2, alpha 2 Subunit (AP2A2))

Alternative Name: AP2A2 / HIP9

Background: The AP2A1 and AP2A2 represent the AP2 alpha proteins that are found in the AP2 complex in clathrincoated vesicles. AP2A is also known as Alpha-adaptin A, Adaptor protein complex AP-2 alpha-1 subunit, Clathrin assembly protein complex 2 alpha-A large chain, 100 kDa coated vesicle protein A and Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit. The AP2 complex is a heterotetramer consisting of two large adaptins (alpha or beta), a medium adaptin (mu), and a small adaptin (sigma). The complex is part of the protein coat on the cytoplasmic face of coated vesicles, which link clathrin to receptors in vesicles.Synonyms: 100 kDa coated vesicle protein C, ADTAB, AP-2 complex subunit alpha-2, Adapter-related protein complex 2 alpha-2 subunit, Adaptor protein complex AP-2 subunit alpha-2, Alpha-2-adaptin, Alpha-adaptin C, CLAPA2, HYPJ, Huntingtin yeast partner J, Huntingtin-interacting protein 9, Huntingtin-interacting protein J, KIAA0899, Plasma membrane adaptor HA2/AP2 adaptin alpha C subunit, lathrin assembly protein complex 2 alpha-C large chain

Gene ID: 161

NCBI Accession: NP_036437

UniProt: O94973

Pathways: EGFR Signaling Pathway, Neurotrophin Signaling Pathway, EGFR Downregulation, SARS-CoV-2 Protein Interactome