WRNIP1 antibody (Internal Region)

Catalog No. ABIN401271

The Rabbit Polyclonal anti-WRNIP1 antibody (ABIN401271) specifically detects WRNIP1 in WB and EIA. The antibody is reactive with Human, Mouse, Rat and Monkey samples.

Quick Overview for WRNIP1 antibody (Internal Region) (ABIN401271)

Target: WRNIP1 (Werner Helicase Interacting Protein 1 (WRNIP1))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WRNIP1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-WRNIP1 Antibody

Binding Specificity: Internal Region

Specificity: This antibody detects WHIP1. Cross reactivity with WHIP2 protein (isoform2) from human sources is expected based on high homology (BLAST).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Monkey (100 %)Species reactivity (tested):Human.

Purification: Immunoaffinity chromatography

Immunogen: Synthetic peptide corresponding to an internal region of the WHIP1 protein

Isotype: IgG

Application Details

Application Notes: ELISA: 1: 10,000 - 1: 40,000. Western blot: 1: 500 - 1: 2,000, detects a band of approximately 96.0 kDa (predictedmolecular weight: 72.2 kDa).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.10 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Target Details for WRNIP1

Target: WRNIP1 (Werner Helicase Interacting Protein 1 (WRNIP1))

Alternative Name: ATPase WRNIP1

Background: Werner's syndrome is a rare autosomal recessive disorder characterized by premature aging. Werner helicase interacting protein 1 (WHIP) interacts with the N-terminal portion of Werner protein, which contains an exonuclease domain. This protein shows homology to replication factor C family proteins, and is conserved from E. coli to human. Studies in yeast suggest that this gene product may influence the aging process. A second isoform exists (WHIP2).Synonyms: WHIP, WRNIP1, Werner helicase-interacting protein 1

Gene ID: 56897

NCBI Accession: NP_064520

UniProt: Q96S55