NCAPH
Reactivity: Human, Rat, Mouse, Guinea Pig, Cow, Dog, Monkey, Horse, Rabbit, Pig, Bat
WB
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: 1/8000 - 1/35000. Western Blot: 1/500 - 1/2000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.93 mg/mL (by UV absorbance at 280 nm)
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
-20 °C
Target
NCAPH
(Non-SMC Condensin I Complex, Subunit H (NCAPH))
NCAPH antibody, si:dkeyp-86b9.4 antibody, zgc:158618 antibody, BRRN1 antibody, CAP-H antibody, brrn1 antibody, cap-h antibody, A730011O11Rik antibody, Brrn1 antibody, HCAP-H antibody, mKIAA0074 antibody, non-SMC condensin I complex subunit H antibody, non-SMC condensin I complex, subunit H antibody, condensin complex subunit 2 antibody, non-SMC condensin I complex subunit H S homeolog antibody, NCAPH antibody, ncaph antibody, LOC708975 antibody, ncaph.S antibody, Ncaph antibody
Background
BRNN1, also called Barren, Condensin subunit 2, Barren homolog protein 1, Chromosomeassociated protein H, hCAP-H, and XCAP-H homolog, is encoded by a gene that is a member of the barr gene family. Brnn1 is the regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. The condensin complex contains the SMC2L1 and SMC4L1 heterodimer, and three non SMC subunits that probably regulate the complex: BRRN1/CAPH, CNAP1/CAPD2 and CAPG. BRRN1 has both a nuclear and cytoplasmic localization. In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated with chromatin. A subpopulation of the complex however remains associated with chromosome foci in interphase cells. During mitosis, most of the condensin complex is associated with the chromatin. At the onset of prophase, the regulatory subunits of the complex are phosphorylated by CDC2 leading to condensin's association with chromosome arms and to chromosome condensation. Dissociation from chromosomes is observed in late telophase. BRRN1 is widely expressed at low level in most proliferating cells. CDC2 phosphorylates BRRN1. Its phosphorylation, as well as that of CNAP1 and CAPG subunits, activates the condensin complex and is required for chromosome condensation.Synonyms: BRRN, BRRN1, CAPH, Chromosome-associated protein H, KIAA0074