NCAPH antibody (AA 440-457)

Catalog No. ABIN401274

The Rabbit Polyclonal anti-NCAPH antibody (ABIN401274) specifically detects NCAPH in WB and EIA. The antibody is reactive with Human and Chimpanzee samples.

Quick Overview for NCAPH antibody (AA 440-457) (ABIN401274)

Target: NCAPH (Non-SMC Condensin I Complex, Subunit H (NCAPH))

Reactivity: Human, Chimpanzee

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCAPH antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-NCAPH Antibody

Binding Specificity: AA 440-457

Specificity: This antibody reacts against BRRN1 protein.

Cross-Reactivity (Details): Species reactivity (expected):Chimpanzee, Mouse, Rat, Canine, Chicken.
Species reactivity (tested):Human.

Purification: Immunoaffinity chromatography

Immunogen: Synthetic peptide corresponding to amino acids 440-457 of Human BRRN-1

Isotype: IgG

Application Details

Application Notes: ELISA: 1/8000 - 1/35000. Western Blot: 1/500 - 1/2000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.93 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for NCAPH

Target: NCAPH (Non-SMC Condensin I Complex, Subunit H (NCAPH))

Alternative Name: NCAPH

Background: BRNN1, also called Barren, Condensin subunit 2, Barren homolog protein 1, Chromosomeassociated protein H, hCAP-H, and XCAP-H homolog, is encoded by a gene that is a member of the barr gene family. Brnn1 is the regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. The condensin complex contains the SMC2L1 and SMC4L1 heterodimer, and three non SMC subunits that probably regulate the complex: BRRN1/CAPH, CNAP1/CAPD2 and CAPG. BRRN1 has both a nuclear and cytoplasmic localization. In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated with chromatin. A subpopulation of the complex however remains associated with chromosome foci in interphase cells. During mitosis, most of the condensin complex is associated with the chromatin. At the onset of prophase, the regulatory subunits of the complex are phosphorylated by CDC2 leading to condensin's association with chromosome arms and to chromosome condensation. Dissociation from chromosomes is observed in late telophase. BRRN1 is widely expressed at low level in most proliferating cells. CDC2 phosphorylates BRRN1. Its phosphorylation, as well as that of CNAP1 and CAPG subunits, activates the condensin complex and is required for chromosome condensation.Synonyms: BRRN, BRRN1, CAPH, Chromosome-associated protein H, KIAA0074

Gene ID: 23397

NCBI Accession: NP_056156

UniProt: Q15003