LIM Domain Kinase 1 antibody (AA 630-647)

Catalog No. ABIN401293

This Rabbit Polyclonal antibody specifically detects LIM Domain Kinase 1 in WB and EIA. It exhibits reactivity toward Human, Mouse, Rat, Chimpanzee and Orang-Utan.

Quick Overview for LIM Domain Kinase 1 antibody (AA 630-647) (ABIN401293)

Target: LIM Domain Kinase 1 (LIMK1)

Reactivity: Human, Mouse, Rat, Chimpanzee, Orang-Utan

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIM Domain Kinase 1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-LIM Domain Kinase 1 Antibody

Binding Specificity: AA 630-647

Specificity: This antibody reacts to LIM kinase protein.

Cross-Reactivity (Details): Species reactivity (expected):Chimpanzee, Orangutan, Mouse, Rat.
Species reactivity (tested):Human.

Purification: Affinity Purified

Immunogen: Synthetic peptide corresponding aa 630-647 of human LIM kinase protein

Isotype: IgG

Application Details

Application Notes: ELISA: 1/8000 - 1/36000. Western Blot: 1/500 - 1/2000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for LIM Domain Kinase 1

Target: LIM Domain Kinase 1 (LIMK1)

Alternative Name: LIMK1

Background: LIM Kinase is also known as LIM-domain containing protein kinase, LIMK-1 and LIMK. There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase 1 and LIM kinase 2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is likely to be a component of an intracellular signaling pathway and may be involved in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. Two splice variant have been identified. LIM kinase has a cytoplamic localization.Synonyms: EC=2.7.11.1, LIM domain kinase 1, LIMK, LIMK-1

Gene ID: 3984

NCBI Accession: NP_001191355

UniProt: P53667

Pathways: Caspase Cascade in Apoptosis, Regulation of Cell Size, CXCR4-mediated Signaling Events