CDC16 antibody (pThr580)

Catalog No. ABIN401306

The Rabbit Polyclonal anti-CDC16 antibody is suitable to detect CDC16 in samples from Human. It has been validated for WB and EIA.

Quick Overview for CDC16 antibody (pThr580) (ABIN401306)

Target: CDC16 (Cell Division Cycle 16 Homolog (S. Cerevisiae) (CDC16))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC16 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-CDC16 Antibody

Binding Specificity: AA 575-588, pThr580

Specificity: This antibody is specific for phosphorylated APC6 protein at the pT580 residue.

Purification: Affinity chromatography

Immunogen: Synthetic peptide corresponding to amino acids 575-588 of Human Apc6 protein

Isotype: IgG

Application Details

Application Notes: ELISA: 1/1,000 - 1/10,000. Western Blot: 1/500 - 1/3,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.97 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for CDC16

Target: CDC16 (Cell Division Cycle 16 Homolog (S. Cerevisiae) (CDC16))

Alternative Name: CDC16 / APC6

Background: APC6 (also known as Cell division cycle protein 16 homolog (CDC16Hs), anaphase promoting complex subunit 6, and cyclosome subunit 6) is a component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C is composed of at least 11 subunits. APC6 interacts with PPP5C and CDC20. This protein colocalizes with CDC27 to the centrosome at all stages of the cell cycle and to the mitotic spindle. Splice variants have been described for this protein.Synonyms: ANAPC6, Anaphase-promoting complex subunit 6, CDC16Hs, Cell division cycle protein 16 homolog, Cyclosome subunit 6

Gene ID: 8881

NCBI Accession: NP_001072113

UniProt: Q13042

Pathways: Regulation of Actin Filament Polymerization