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CCCTC-Binding Factor (Zinc Finger Protein)-Like (CTCFL) (AA 9-26) antibody

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Synonyms CTCFL, BORIS, CT27, CTCF-T, HMGB1L1, dJ579F20.2, Boris, OTTMUSG00000016680
AA 9-26
(7), (4), (1), (1), (1), (1)
Chimpanzee, Human
(44), (26), (24), (1)
(48), (2), (1), (1)
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Enzyme Immunoassay (EIA), Western Blotting (WB)
(38), (11), (10), (10), (8), (3), (2), (2), (2), (1)
Pubmed 2 references available
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Quantity 0.1 mg
Shipping to United States ( )
Availability Will be delivered in 6 to 8 Business Days
Immunogen Synthetic peptide corresponding aa 9-26 of human BORIS protein
Isotype IgG
Specificity This antibody is directed against the BORIS protein.
Cross-Reactivity (Details) Species reactivity (expected):Chimpanzee.
Species reactivity (tested):Human.
Purification Affinity chromatography
Alternative Name CTCFL / BORIS (CTCFL Antibody Abstract)
Background BORIS (Brother of the Regulator of Imprinted Sites) also known as CCCTC-binding factor-like protein, is normally only expressed in the testis and expressed in a mutually exclusive manner with CTCF during male germ cell development. However, previous studies have shown that BORIS is abnormally activated in a wide range of human cancers. Expression of BORIS in normally BORIS-negative cells promotes cell growth that may lead to transformation. BORIS maps to the cancer-associated amplification region thought to contain an oncogene or dominant-immortalizing gene. BORIS is a candidate protein for the epigenetic reprogramming factor acting in the male germ line. BORIS is found in both the nucleus and cytoplasm.Synonyms: Brother of the regulator of imprinted sites, CCCTC-binding factor, CT27, CTCF paralog, CTCF-T, CTCF-like protein, Cancer/testis antigen 27, Transcriptional repressor CTCFL, Zinc finger protein CTCF-T
Gene ID 140690
NCBI Accession NP_542185
UniProt Q8NI51
Application Notes ELISA: 1/2,000 - 1/10,000. Western Blot: 1/200 - 1/2,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.28 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
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 image for anti-CCCTC-Binding Factor (Zinc Finger Protein)-Like (CTCFL) (AA 9-26) antibody (ABIN401325) anti-CCCTC-Binding Factor (Zinc Finger Protein)-Like (CTCFL) (AA 9-26) antibody
Background publications Klenova, Morse, Ohlsson et al.: "The novel BORIS + CTCF gene family is uniquely involved in the epigenetics of normal biology and cancer." in: Seminars in cancer biology, Vol. 12, Issue 5, pp. 399-414, 2002 (PubMed).

Loukinov, Pugacheva, Vatolin et al.: "BORIS, a novel male germ-line-specific protein associated with epigenetic reprogramming events, shares the same 11-zinc-finger domain with CTCF, the insulator protein involved in reading imprinting marks in the soma." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 10, pp. 6806-11, 2002 (PubMed).

Catalog No. ABIN401325
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