CD151 antibody (pSer30)

Catalog No. ABIN401337

The Rabbit Polyclonal anti-CD151 antibody (ABIN401337) specifically detects CD151 in WB and EIA. The antibody is reactive with Saccharomyces cerevisiae samples.

Quick Overview for CD151 antibody (pSer30) (ABIN401337)

Target: CD151

Reactivity: Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD151 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-CD151 Antibody

Binding Specificity: AA 26-35, pSer30

Specificity: This antibody is directed against the phosphorylated form of Saccharomyces cerevisiae Mer2 protein at the pS30 residue.

Purification: Affinity chromatography

Immunogen: Synthetic peptide corresponding to amino acids 26-35 of Saccharomyces cerevisiae Mer2 protein

Isotype: IgG

Application Details

Application Notes: ELISA: 1/5,000 - 1/25,000. Western Blot: 1/1,000 - 1/10,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.77 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for CD151

Target: CD151

Alternative Name: MER2

Background: Mer2 (also known as meiotic recombination 2 protein) is a chromosomal protein that is critical for meiotic recombination and progression. It is phosphorylated at two serine residues, S30 and S271 by the yeast Cdk1 cyclin- dependent kinase homolog. This phosphorylation is S-phase specific, and thus has the potential to be a specific assay for S-phase cyclin-dependent kinases. Moreover, there are hints that the phosphorylation may be a mark of replication fork passage, which would indicate that Sphase CDK associates with the replication fork.Synonyms: J1462, Meiotic recombination 2 protein, REC107, YJR021C

Gene ID: 853478

NCBI Accession: NP_012555

UniProt: P21651