ECT2 antibody (pThr790)

Catalog No. ABIN401345

The Rabbit Polyclonal anti-ECT2 antibody has been validated for WB, IP and EIA. It is suitable to detect ECT2 in samples from Human, Mouse, Rat, Dog, Zebrafish (Danio rerio), Chicken and Chimpanzee.

Quick Overview for ECT2 antibody (pThr790) (ABIN401345)

Target: ECT2 (Epithelial Cell Transforming Sequence 2 Oncogene (ECT2))

Reactivity: Human, Mouse, Rat, Dog, Zebrafish (Danio rerio), Chicken, Chimpanzee

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ECT2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Product Details anti-ECT2 Antibody

Binding Specificity: AA 785-795, pThr790

Specificity: This antibody is directed against the phosphorylated form of ECT2 protein at the pT790 residue.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Zebrafish, Chimpanzee, Chicken, Canine.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Synthetic peptide corresponding to amino acids 785-795 of human ECT2 protein

Isotype: IgG

Application Details

Application Notes: ELISA: 1/10,000 - 1/40,000. Western Blot: 1/1,000 - 1/4,000. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.31 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for ECT2

Target: ECT2 (Epithelial Cell Transforming Sequence 2 Oncogene (ECT2))

Alternative Name: ECT2

Background: ECT2, also known as epithelial cell transforming sequence 2 oncogene, was originally isolated as a transforming gene from epithelial cells. ECT2 catalyzes guanine nucleotide exchange on the small GTPases, RhoA, Rac1, and Cdc42. ECT2 may be phosphorylated during G2 and M phases, and phosphorylation may be required for its exchange activity. Unlike other known guanine nucleotide exchange factors for Rho GTPases, ECT2 exhibits nuclear localization in interphase, spreads throughout the cytoplasm in prometaphase, and is condensed in the midbody during cytokinesis. Expression of dominant-negative ECT2 or microinjection of affinity-purified anti-ECT2 antibody into interphase cells strongly inhibits cytokinesis. These results suggest that ECT2 is an important link between the cell cycle machinery and Rho signaling pathways involved in the regulation of cell division. Phosphorylation at T790 or S375 significantly affects the catalytic activity of ECT2.Synonyms: Epithelial cell-transforming sequence 2 oncogene

Gene ID: 1894

NCBI Accession: NP_060568

UniProt: Q9H8V3

Pathways: Neurotrophin Signaling Pathway, Cell-Cell Junction Organization