ECT2 antibody (N-Term)

Catalog No. ABIN185018

The Goat Polyclonal anti-ECT2 antibody is suitable to detect ECT2 in samples from Human. It has been validated for ELISA, FACS and IF.

Quick Overview for ECT2 antibody (N-Term) (ABIN185018)

Target: ECT2 (Epithelial Cell Transforming Sequence 2 Oncogene (ECT2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ECT2 antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Grade: Verified

Product Details anti-ECT2 Antibody

Binding Specificity: N-Term

Purpose: ECT2

Sequence: AENSVLTSTT GRT

Specificity: This antibody is expected to recognise NP_001245244.1 (iso a), NP_060568.3 (iso b), NP_001336023.1 (iso c), NP_001336026.1 (iso d), NP_001336030.1 (iso e), NP_001336031.1 (iso f), NP_001336032.1 (iso g)

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence AENSVLTSTTGRT-C, from the N Terminus of the protein sequence according to NP_060568.3.

Isotype: IgG

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of A431 and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended conc

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ECT2

Target: ECT2 (Epithelial Cell Transforming Sequence 2 Oncogene (ECT2))

Alternative Name: ECT2

Background: ECT2, epithelial cell transforming sequence 2 oncogene, FLJ10461, epithelial cell transforming sequence 2 oncogene protein

Gene ID: 1894

NCBI Accession: NP_060568

Pathways: Neurotrophin Signaling Pathway, Cell-Cell Junction Organization