TAF1 antibody (C-Term)

Catalog No. ABIN401389

This Rabbit Polyclonal antibody specifically detects TAF1 in WB and EIA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for TAF1 antibody (C-Term) (ABIN401389)

Target: TAF1 (TAF1 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 250kDa (TAF1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TAF1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-TAF1 Antibody

Binding Specificity: C-Term

Specificity: This antibody is directed against TAF1 protein.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Synthetic peptide corresponding to amino acids near the carboxyl terminus of human TAF1

Isotype: IgG

Application Details

Application Notes: ELISA: 1/10,000 - 1/50,000. Western Blot: 1/100 - 1/2,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.15 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for TAF1

Target: TAF1 (TAF1 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 250kDa (TAF1))

Alternative Name: TAF1

Background: Transcription Initiation Factor TFIID Subunit 1 (TAF1) is the largest component and core scaffold of the TFIID basal transcription factor complex, which also includes TATA-binding protein (TBP) and a variety of TBP-associated factors. TFIID nucleates the formation of transcription preinitiation complexes and plays a key role in the regulation of gene expression by RNA polymerase II. TAF1 possesses DNA-binding activity and also contains novel N- and C-terminal Ser/Thr kinase domains which can auto-phosphorylate or trans-phosphorylate other transcription factors. For example, TAF1 interacts with the C-terminus of TP53 and phosphorylates the T55 residue, leading to MDM2-mediated degradation of TP53. TAF1 also catalyzes Ser phosphorylation of general transcription factor IIA (GTF2A1) and IIF (GTF2F1). The retinoblastoma tumor suppressor protein, RB1, interacts with the N-terminal domain of TAF1 and inhibits its intrinsic kinase activity. TAF1 is essential for progression through the G1 phase of the cell cycle and has been reported to be indispensable for expression of 18 % of mammalian genes.Synonyms: BA2R, CCG1, CCGS, Cell cycle gene 1 protein, TAFII250, TBP-associated factor 250 kDa, Transcription initiation factor TFIID 250 kDa subunit, Transcription initiation factor TFIID subunit 1

Gene ID: 6872

NCBI Accession: NP_004597

UniProt: P21675